Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances  on Pseudomonas aeruginosa Isolated from Chronic Wounds

Akram  Etemadinia; Amir  Seyfoori; Abbas  Rahimi Foroushani; Ramin  Mazaheri Nezhad Fard; Ronak  Bakhtiari

doi:10.18502/ajmb.v14i4.10487

Akram Etemadinia Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
Amir Seyfoori Department of Biomaterials and Tissue Engineering, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Abbas Rahimi Foroushani Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
Ramin Mazaheri Nezhad Fard Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
Ronak Bakhtiari Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran

DOI: https://doi.org/10.18502/ajmb.v14i4.10487

Keywords: Biofilms, Microgels, Pseudomonas aeruginosa, Real-time PCR

Abstract

Background: Biofilm formation helps Pseudomonas ‎aeruginosa (P. ‎aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropyl-acrylamide microgels (PNIPAM) on P. aeruginosa.

Methods: Totally‎, ‎100 P. aeruginosa strains were isolated from chronic wound infections‎. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates.

Results: Significant decreases were seen in lasR expression in EDTA-treated samples‎. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased ‎expression of PA2714 was seen in samples treated with xylitol with no significance. ‎

Conclusion: The PNIPAM containing xylitol ‎or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.