Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds

Authors

  • Akram Etemadinia Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
  • Amir Seyfoori Department of Biomaterials and Tissue Engineering, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
  • Abbas Rahimi Foroushani Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
  • Ramin Mazaheri Nezhad Fard Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran
  • Ronak Bakhtiari Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran

DOI:

https://doi.org/10.18502/ajmb.v14i4.10487

Keywords:

Biofilms, Microgels, Pseudomonas aeruginosa, Real-time PCR

Abstract

Background: Biofilm formation helps Pseudomonas ‎aeruginosa (P. ‎aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropyl-acrylamide microgels (PNIPAM) on P. aeruginosa.

 

Methods: Totally‎, ‎100 P. aeruginosa strains were isolated from chronic wound infections‎. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates.

 

Results: Significant decreases were seen in lasR expression in EDTA-treated samples‎. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased ‎expression of PA2714 was seen in samples treated with xylitol with no significance. ‎

 

Conclusion: The PNIPAM containing xylitol ‎or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.

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Published

2022-09-03

Issue

Volume (14) issue (4)

Section

Articles