Effect of Cuminum Cyminum and Caraway Essential Oil on Bacillus Cereus Inoculated in Sterilized Milk

Bahareh  Jafari-Bojd; Mohammad Rahnama; Majid  Alipour-Eskandani; Ali  Afshari-Moghadam; Dariush Saadati; Saeedeh Saeedi

doi:10.18502/jzms.v4i4.8746

Bahareh Jafari-Bojd DVM Graduated, School of Veterinary Medicine, University of Zabol, Zabol, Iran
Mohammad Rahnama Associated Professor of Food Quality Control and Hygiene, School of Veterinary Medicine, University of Zabol, Zabol, Iran
Majid Alipour-Eskandani Associated Professor of Food Quality Control and Hygiene, School of Veterinary Medicine, University of Zabol, Zabol, Iran
Ali Afshari-Moghadam Instructor, Department of Nutrition and Animal Breeding, School of Veterinary Medicine, University of Zabol, Zabol, Iran
Dariush Saadati Associate Professor of Veterinary Epidemiology, School of Veterinary Medicine, University of Zabol, Zabol, Iran
Saeedeh Saeedi MSc, Biotechnology Research Institute (Biosenter), University of Zabol, Zabol, Iran

DOI: https://doi.org/10.18502/jzms.v4i4.8746

Keywords: Bacillus cereus, Cuminium cyminum, Caraway, Sterilized milk

Abstract

Background: In recent years, particular attention has been paid to the use of natural preservatives in foods instead of chemical ones.

Methods: In this study, the antimicrobial activity of Cuminum cyminum and Caraway essential oil on Bacillus cereus at 10°C in sterilized milk was investigated. The Bacillus cereus Bacterium was recovered in a nutritious Meal culture medium. Subsequently, 10³ bacteria per cc in milk were selected and into utensil milk those were added to autoclaved before that time. Then, Concentrations of 0, 0.03, 0.06, 0.1, 0.2, 1 and 2 percent of essential oil of Cuminum cyminum plant and of 0, 0.03, 0.06, 0.1, 0.2, 1, 1.5 and 2 percent of the essential oil of the caraway plant was added to the sterilized milk container. Then the study of bacterial condition was done on days 0, 1, 3, 6, 9, 12, 15, 18 and 21 by spread plate count. Then, according to the logarithm of the obtained data, repeated measures of ANOVA.

Results: Based on the results, it was found that essential oils of cumin and caraway had an inhibitory effect on growth of Bacillus cereus compared to control group (p value < 0.05).

Conclusion: In this research, essential oils of Cumin and caraway showed suitable antibacterial effects against growth of Bacillus cereus in milk. The results showed that Cumin is more effective than caraway.