Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection

Hamed Rahnavard

doi:10.18502/jnfs.v7i4.11061

Hamed Rahnavard School of Agricultural Sciences and Food Industries, Ardestan Branch of Islamic Azad University.

DOI: https://doi.org/10.18502/jnfs.v7i4.11061

Keywords: Astaxanthin; High performance liquid chromatography; Fish; Upper limit of quantification (ULOQ); lower limit of quantification (LLOQ)

Abstract

Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout.

Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v.

Results: Despite the optimal conditions, analytical property of the method containing lower limit of quantitation (2.00 ng g-1) and upper limit of quantitation (1000 ng g-1), linearity (r₂=0.992), and recovery (103%) were acceptable. The results showed the range of 105 ± 0.09 - 875 ± 0.020 ng g-1.

Conclusion: The method was successfully used to quantify the target analytes in all trout samples.