Association of MTHFR, BMP4, TGFA and IRF6 Polymorphisms with Non-Syndromic  Cleft lip and Palate in North Indian Patients

Kapil Kumar  Avasthi; Amit  Agarwal; Sarita  Agarwal

doi:10.18502/ajmb.v14i2.8879

Kapil Kumar Avasthi Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS) Lucknow 226014, India
Amit Agarwal Department of Burn and Plastic Surgery, Vivekananda Polyclinic and Institute of Medical Sciences (VPIMS), Lucknow 226007, India
Sarita Agarwal Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS) Lucknow 226014, India

DOI: https://doi.org/10.18502/ajmb.v14i2.8879

Keywords: BMP4, Indian population, IRF6, MTHFR, NSCL/P, TGFA

Abstract

Background: Non-Syndromic Cleft Lip and Palate (NSCL/P) is a multifactorial birth defect. The world-wide prevalence of NSCL/P is 1 in 1000 live births; it differs with race, ethnicity and gender. The aim of the present study was to find out the status of candidate gene polymorphisms in NSCL/P cases and its association in phenotype of the patients.

Methods: We have screened five polymorphisms in four candidate genes MTHFR (rs1801133, rs1801131) BMP4 (rs17563), TGFA (rs1146297) and IRF6 (rs2235371) by restriction fragment length polymorphism and results were validated by Sanger sequencing. Our dataset consists of 200 NSCL/P cases and 200 healthy controls from the Indian population. Statistical data analysis was performed by SPSS software.

Results: MTHFR (rs1801133), BMP4 (rs175563) and TGFA (rs11466297) gene polymorphisms showed significant association with NSCL/P and act as a risk factor in the Indian population (p=<0.05). However, MTHFR (rs1801131), and IRF6 (rs2235371) gene polymorphisms did not show significant association with NSCL/P in the Indian population.

Conclusion: The result of the study suggests an association between MTHFR (rs1801133), BMP4 (rs175563) and TGFA (rs11466297) polymorphisms with NSCL/P in Indian population.