Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells

Miganoosh  Simonian; Mozhan  Haji Ghaffari; Ali  Salimi; Ebrahim Mirzadegan; Niloufar  Sadeghi; Nasim  Ebrahimnezhad; Ghazaleh  Fazli; Ramina  Fatemi; Ali-Ahmad  Bayat; Saeideh Milani; Babak  Negahdari; Hodjattallah  Rabbani

doi:10.18502/ajmb.v14i1.8168

Miganoosh Simonian Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mozhan Haji Ghaffari Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Ali Salimi Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Ebrahim Mirzadegan Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Niloufar Sadeghi Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Nasim Ebrahimnezhad Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Ghazaleh Fazli Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Ramina Fatemi Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Ali-Ahmad Bayat Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Saeideh Milani Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Babak Negahdari Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Hodjattallah Rabbani Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

DOI: https://doi.org/10.18502/ajmb.v14i1.8168

Keywords: Breast cancer, Flow cytometry, Monoclonal antibody, Sortilin

Abstract

Background: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells.

Methods: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines.

Results: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines.

Conclusion: Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.