Silencing SALL-4 Gene by Transfecting Small Interfering RNA with Targeted Aminoglycoside-Carboxyalkyl Polyethylenimine Nano-Polyplexes Reduced Migration of MCF-7 Breast Cancer Cells

Somaye   Noruzi; Mehran   Vatanchian; Amir  Azimian; Arash  Niroomand; Reza   Salarinia; Fatemeh   Oroojalian

doi:10.18502/ajmb.v13i1.4580

Somaye Noruzi
Mehran Vatanchian
Amir Azimian
Arash Niroomand
Reza Salarinia
Fatemeh Oroojalian

DOI: https://doi.org/10.18502/ajmb.v13i1.4580

Keywords: Breast neoplasms, Gene transfer techniques, MCF-7 cells, Transcription factors

Abstract

Background: The application of non-viral systems for delivering genes to cells is becoming a very interesting issue, especially in the treatment of neoplasms such as Breast Cancer (BC). Polymer-based non-viral systems are safe and feasible gene carriers to be used in targeted cancer therapy. SALL4 gene encodes a transcription factor and is overexpressed in some cancers.

Methods: In this study, carboxyalkylated-PEI25 (25 kDa) was used to deliver plasmids expressing SALL4-siRNA into MCF-7 cells. DLS and AFM were applied to determine the size of nanoparticles. The MTT method was used to assess cytotoxicity, and the efficiency of transfection was confirmed both qualitatively and quantitatively. Finally, the effect of silencing SALL4 was investigated on the migration of MCF7 cells using the scratch test.

Results: The results showed that transferring the SALL4-siRNA using PEI25G10C50 reduced the expression of the corresponding transcription factor by 14 folds which attenuated the migration of MCF-7 cells by 58%.

Conclusion: In conclusion, PEI25G10C50 can serve as an effective gene delivery system for treating BC by targeting SALL-4.