Comparison of Antifungal Properties of Gold, Silver, and Selenium Nanoparticles Against Amphotericin B-Resistant Candida glabrata Clinical Isolates

Ensieh   Lotfali; Hossein   Toreyhi; Kamyab   Makhdoomi Sharabiani; Azam  Fattahi; Amirali   Soheili; Reza  Ghasemi; Mahyar   Keymaram; Yasaman   Rezaee; Sayna Iranpanah

doi:10.18502/ajmb.v13i1.4578

Ensieh Lotfali
Hossein Toreyhi
Kamyab Makhdoomi Sharabiani
Azam Fattahi
Amirali Soheili
Reza Ghasemi
Mahyar Keymaram
Yasaman Rezaee
Sayna Iranpanah

DOI: https://doi.org/10.18502/ajmb.v13i1.4578

Keywords: Antifungal agents, Candida glabrata, Nanoparticles, Silver

Abstract

Background: The present study aimed to investigate the antifungal activity of Nanoparticles (NPs) against amphotericin B-resistant Candida glabrata (C. glabrata) strains.

Methods: Twelve resistant (C. glabrata) strains were isolated from archived clinical isolates. Antifungal activity was conducted according to Clinical and Laboratory Standards Institute’s (CLSI) guidelines, document M27-A3/S4. The Scanning Electron Microscope (SEM) was used to observe the morphological changes of strains exposed to each nanoparticle.

Results: Minimum Inhibitory Concentration (MIC) of nanoparticles of all strains was in the concentration range of 0.125 to 0.5 µg/Ml. The synthesized Ag-NPs showed superior antifungal activity against (C. glabrata) compared to Se-NPs and Au-NPs. The scanning electron microscope images revealed the difference in the fungal morphology between the untreated and treated fungi with nanoparticles.

Conclusion: The Ag-NPs, followed by Se-NPs synthesized, revealed significant antifungal activity against resistance regardless of their antifungal-resistant mechanisms.