Therapeutic Effects of Nanochelating-Based Copper Nanoparticles on Burn Wound  Healing in Mouse Model

Hossein  Rezvan; Seyed Masoud  Zolhavarieh; Alireza  Nourian; Elham  Bayat; Somayeh  Kalanaky; Saideh  Fakharzadeh; Maryam  Hafizi; Mohammad Hassan  Nazaran; Sahar  Hamoonnavard

doi:10.18502/ajmb.v17i1.17672

Hossein Rezvan Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Seyed Masoud Zolhavarieh Department of Clinical Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Alireza Nourian Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Elham Bayat Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Somayeh Kalanaky Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran
Saideh Fakharzadeh Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran
Maryam Hafizi Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran
Mohammad Hassan Nazaran Department of Research and Development, Sodour Ahrar Shargh Company, Tehran, Iran
Sahar Hamoonnavard Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran

DOI: https://doi.org/10.18502/ajmb.v17i1.17672

Keywords: Anti-inflammatory agents, Burns, Copper, Dermatology, Wound healing

Abstract

Background: The aim of the present study was to investigate the potential of Nanochelating-based copper to accelerate the wound healing process and prevent infection in burn wounds.

Methods: Six to eight-week- old female BALB/c mice were burned with a 1 cm² heated copper plate on the left flank and then divided into four treatment groups, treated with C8 (nanochelating-based CuNPs), cold cream (supplementary materials) as a control drug, Silver Sulfadiazine and no treatment, respectively. Skin tissue samples were taken from the mice on days 0, 3, 8, 15 and 24. One piece was fixed in 10% neutral buffered formalin for pathological examination and the others were stored at -80°C until used for pro-inflammatory and growth factor gene expression.

Results: The healing process in the group treated with 10 mg/ml C8 was significantly faster, and the survival rate of the mice in this group was significantly higher than in the other groups. The pro-inflammatory genes were expressed and down-regulated earlier in the C8 treated mice. Histopathology confirmed the higher cure rate in the group treated with 10 mg/ml C8 compared to other control groups.

Conclusion: C8 has beneficial effects on the healing of burn wounds and the effective dose of this compound should be further investigated. The present study demonstrates the anti-inflammatory properties of nano-chelate-based copper particles' on mouse skin burns. This research opens up new possibilities in dermatology and burn therapy and highlights the potential of copper-based formulations in the treatment of burn injuries