Lippia multiflora Leaves Extracts Enhance Cefotaxime Bactericidal Effects and  Quench the Biofilm Formation in Methicillin-Resistant Staphylococcus aureus ATCC 43300

Ablassé  Rouamba; Djaouratou  Badini; Eli  Compaoré; Vincent  Ouédraogo; Martin  Kiendrebeogo

doi:10.18502/ajmb.v16i3.15746

Ablassé Rouamba Laboratory of Applied Biochemistry and Chemistry, Department of Biochemistry-Microbiology, UFR SVT, Université Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou 03, Burkina Faso
Djaouratou Badini Laboratory of Applied Biochemistry and Chemistry, Department of Biochemistry-Microbiology, UFR SVT, Université Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou 03, Burkina Faso
Eli Compaoré Laboratory of Applied Biochemistry and Chemistry, Department of Biochemistry-Microbiology, UFR SVT, Université Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou 03, Burkina Faso
Vincent Ouédraogo Laboratory of Applied Biochemistry and Chemistry, Department of Biochemistry-Microbiology, UFR SVT, Université Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou 03, Burkina Faso
Martin Kiendrebeogo Laboratory of Applied Biochemistry and Chemistry, Department of Biochemistry-Microbiology, UFR SVT, Université Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou 03, Burkina Faso

DOI: https://doi.org/10.18502/ajmb.v16i3.15746

Keywords: Anti-Bacterial Agents, Antioxidants, Biofilms, Lippia, Methicillin-resistant staphylococcus aureus, Methylene chloride, Plant extracts

Abstract

Background: The emergence of the multidrug-resistant bacteria strain has become a global world crisis. This study was designed to evaluate the antibiofilm and synergistic effects of Lippia multiflora leaf extracts on the activity of cefotaxime against the methicillin-resistant Staphylococcus aureus (S. aureus).

Methods: The synergistic effect of methanol and dichloromethane extracts on the bactericidal activity of cefotaxime was determined by using the antibiotic susceptibility test on agar medium. The antibiofilm activity of the extracts was measured by using the crystal violet method. The antioxidant potential of the extracts was assessed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reduction Activity Potential (FRAP) methods. The main secondary metabolites groups were analyzed by using different standard analytical tests. The total phenolics and total flavonoids were quantified spectrophotometrically.

Results: The methanol extract (final concentration of 100 µg/ml) inhibited the formation of bacterial biofilm more than salicylic acid (p<0.05). All extracts combined with cefotaxime (20 µg and 200 µg) showed good synergistic bactericidal effect on S. aureus with inhibitory diameters of up to 40 mm. The methanol extract showed higher total phenolics (462.20±10.90 mg EAG/g) and total flavonoids (26.20±0.20 mg EQ/g) contents than the dichloromethane extract (96.70±1.70 mg EAG/g and 8.00±1.20 mg EQ/g). Moreover, the methanol extract showed a higher FRAP reducing power (353.6± 4.17 mmol EQ/g) than the dichloromethane extract (385.3±7.01 mmol EQ/g). Qualitative phytochemical analysis showed the presence of tannins, flavonoids, terpenes and sterols in both extracts.

Conclusion: These data showed that L. multiflora leaves contain effective antibacterial phytomolecules for combating bacterial resistance.