Bactericidal Activity of Serum by Brucella Abortus RB51 Outer Membrane Protein’s  Combined by Brucella Abortus S99 Lipopolysaccharide Induction

Behnam Hajizadeh  Sisakht; Mansoor  Khaledi; Hamed  Afkhami; Saber  Rouhi; Saeed  Sepehrnia; Vahideh  Fanaee; Hannaneh  Karimi; Yalda  Malekzadegan; Javad  Fathi; Mahdi S.  Sadati

doi:10.18502/ajmb.v16i3.15745

Behnam Hajizadeh Sisakht Department of Bacteriology, Faculty of Veterinary, Islamic Azad University, Science & Research Campus, Tehran, Iran
Mansoor Khaledi Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Hamed Afkhami Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
Saber Rouhi Resident of Large Animal Internal Medicine, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Iran
Saeed Sepehrnia Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
Vahideh Fanaee Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences,Shahrekord, Iran
Hannaneh Karimi Department of Microbiology, Rasht Islamic Azad University, Guilan, Iran
Yalda Malekzadegan Department of Microbiology, Saveh University of Medical Sciences, Saveh, Iran
Javad Fathi Department of Medical Bacteriology and Virology, Faculty of Medicine, Shiraz University of Medical Sciences,Shiraz, Iran
Mahdi S. Sadati Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University Tehran-North Branch, Tehran,Iran

DOI: https://doi.org/10.18502/ajmb.v16i3.15745

Keywords: Agar, Brucellosis, Cellular, Combined, Humoral, Immunity, Immunity, Lipopolysaccharides, Vaccines

Abstract

Background: Brucellosis vaccines are designed to induce cellular immunity. An effective brucellosis vaccine could induce both cellular and humoral immunity. Serum Bactericidal Assay (SBA) is an important method for determining vaccine humoral immunity. This study is the first to observe humoral immunity in brucellosis by SBA.

Methods: Extracted Brucella abortus (B. abortus) Lipopolysaccharide (LPS) and Outer Membrane Proteins (OMPs) were injected into rabbits. Group 1 was injected with 25 µg of LPS, Group 2 was injected with 50 µg of OMPs, and Group 3 was injected with 1 ml of combined vaccine, 3 times every 2 weeks. The groups were challenged with B. abortus 544 in the second injection. Sera were separated 2 weeks after the last injection. SBA was performed, and each well was streak-cultured into a plate of Brucella agar. A colony count was done for each plate.

Results: Results have shown, the third injection of the combined vaccine had the highest titer of , and the efficacy of the vaccine was 87.71%.

Conclusion: As a conclusion, the results of this study showed that LPS and OMP's from B. abortus can provide acceptable immunity.