The Protective Effect of N-acetylcysteine against Deltamethrin-Induced  Hepatotoxicity in Mice

Ali  Ameri; Alireza  Rahmati; Shadi  Soroushfar; Mehdi  Lalehzari; Tahereh  Dehghani; Hamed  Haghi-Aminjan; Jebreil  Shamseddin; Mahmoud  Omidi

doi:10.18502/ajmb.v16i2.14859

Ali Ameri Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Alireza Rahmati Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Shadi Soroushfar Student Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Mehdi Lalehzari Trauma Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Tahereh Dehghani Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Hamed Haghi-Aminjan Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran
Jebreil Shamseddin Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Mahmoud Omidi Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

DOI: https://doi.org/10.18502/ajmb.v16i2.14859

Keywords: Deltamethrin, Hepatoprotection, Liver, N-acetylcysteine, Pesticides

Abstract

Background: Exposure to pesticides is of concern to public health officials worldwide. Deltamethrin is a synthetic pyrethroid pesticide which is widely used in agriculture and veterinary medicine. Deltamethrin poisoning is always one of the concerns in medical centers due to the deltamethrin induced hepatotoxicity. This study evaluated the hepatoprotective effects of N-acetylcysteine (NAC) against deltamethrin induced hepatotoxicity in mice

Methods: A total of 40 BALB/c male mice were randomly divided into four groups; the first group was used as a control (0.5 ml normal saline); Groups 2-4 were treated with NAC [160 mg/kg Body Weight (BW)], deltamethrin (50 mg/kg BW), and NAC plus deltamethrin. At 1 and 24 hr after treatment, the animals were sacrificed and blood and liver samples were obtained for analysis and the liver/body ration, hepatic enzymes as Aspartate aminotransferase (AST), Alanine Transaminase (ALT), Alkaline phosphatase (ALP), Lactate dehydrogenase (LDH), Glutathione (GSH) content and Reactive Oxygen Species (ROS) level were measured. For comparison between more than two experimental groups, one-way ANOVA following Tukey test was used by SPSS software.

Results: The deltamethrin significantly increased AST, ALT, ALP, and the level of ROS level at the end of 1 and 24 hr after treatment; while the LDH level and GSH content were decreased. Mice in the deltamethrin treated group had a higher liver/body weight ratio than in other treated groups after 24 hr. On the other hand, NAC in combination with deltamethrin significantly reduced the activities of AST, ALT, ALP, and increased GSH levels.

Conclusion: This study demonstrated that NAC has a hepatoprotective role against deltamethrin-induced toxicity.