Extracellular L-Asparaginase Synthesis Bacillus niacin Isolation, Optimization, and  Characterization from Marine Saltern Sediment Sources

Mugip Rahaman  Abdul Wahab; Thirunavukkarasu  Palaniyandi; John  Wyson; Asha  Sivaji; Swarnakala  Thamada

doi:10.18502/ajmb.v16i1.14170

Mugip Rahaman Abdul Wahab Department of Biotechnology, Dr. M.G.R Educational and Research Institute, Chennai, Tamil Nadu, India
Thirunavukkarasu Palaniyandi Department of Biotechnology, Dr. M.G.R Educational and Research Institute, Chennai, Tamil Nadu, India
John Wyson Department of Food Processing Technology, AMET University, Kanathur, Chennai, Tamil Nadu, India
Asha Sivaji Department of Biochemistry, DKM College for Women, Vellore-632001, Tamil Nadu, India
Swarnakala Thamada Molecular Systematics Laboratory, Zoological Survey of India, Andaman & Nicobar Regional Centre, Port Blair-744 102, Andaman and Nicobar Islands

DOI: https://doi.org/10.18502/ajmb.v16i1.14170

Keywords: Asparagine, Asparaginase, Bacillus niacini, Polymerase chain reaction

Abstract

Background: Asparagine is an amino acid that can be converted into aspartic acid and ammonia by the enzyme L-asparaginase. Some forms of cancer, such Acute Lymphoblastic Leukaemia (ALL) and Non-Hodgkin Lymphoma (NHL), respond well to this enzyme when employed as a chemotherapeutic drug. The purpose of this research was to find bacteria that can manufacture the enzymes L-asparaginasein marine slattern sediment which can be employed in commercial and industrial scale production.

Methods: All of the strains were identified as Bacillus niacini spp. by biochemical and molecular testing. The strain belongs to the Bacillus genus, according to nutritional, biochemical, PCR and 16srRNA sequencing data.

Results: According to the findings of this research, Bacillus niacin spp. have the potential to create a substance that is helpful in a variety of medical applications. The results of this study hint to the possibility that bacteria have the ability to produce antimicrobial compounds, which have the potential to be successful in a wide variety of environments.

Conclusion: Numerous opportunities may arise for researchers interested in utilizing the medical potential of enzyme-producing bacteria if they are successfully isolated and screened from aquatic and terrestrial habitats.