Effect of Intra-ovarian Injection of Mesenchymal Stem Cells or its Conditioned  Media on Repeated OPU-IVEP Outcomes in Jersey Heifers and Its Relationship with  Follicular Fluid Inflammatory Markers

Ali  Sarvari; Amir  Niasari-Naslaji; Abolfazl  Shirazi; Banafsheh  Heidari; Sara  Borjian Boroujeni; Mohammad Hossein  Moradi; Mohammad-Mahdi  Naderi; Bahareh  Behzadi; Mohammad-Mahdi  Mehrazar; Mohammad Mehdi  Dehghan

doi:10.18502/ajmb.v16i1.14167

Ali Sarvari Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Amir Niasari-Naslaji Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abolfazl Shirazi Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Banafsheh Heidari Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
Sara Borjian Boroujeni Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mohammad Hossein Moradi Department of Animal Sciences, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
Mohammad-Mahdi Naderi Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Bahareh Behzadi Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mohammad-Mahdi Mehrazar Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mohammad Mehdi Dehghan Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

DOI: https://doi.org/10.18502/ajmb.v16i1.14167

Keywords: Animals, Biomarkers, Cattle, Conditioned medium, Follicular fluid, Inflammation, Interleu-kin-12, Ovary, Resolvin E1, Stem cell

Abstract

Background: Repeated Ovum Pick Up (OPU) could have a detrimental effect on ovarian function, reducing In Vitro Embryo Production (IVEP). The present study examined the therapeutic effect of adipose–derived Mesenchymal Stem Cells (MSCs) or its Conditioned Medium (ConM) on ovarian trauma following repeated OPU. Resolvin E1 (RvE1) and Interleukin-12 (IL-12) were investigated as biomarkers.

Methods: Jersey heifers (n=8) experienced 11 OPU sessions including 5 pre-treatment and 6 treatment sessions. Heifers received intra-ovarian administration of MSCs or ConM (right ovary) and Dulbecco’s Modified Phosphate Buffer Saline (DMPBS; left ovary) after OPU in sessions 5 and 8 and 2 weeks after session 11. The concentrations of RvE1 and IL-12 in follicular fluid was evaluated on sessions 1, 5, 6, 9, and 4 weeks after session 11. Following each OPU session, the IVEP parameters were recorded.

Results: Intra-ovarian administration of MSCs, ConM, and DMPBS did not affect IVEP parameters (p>0.05). The concentration of IL-12 in follicular fluid increased at the last session of pre-treatment (Session 5; p<0.05) and remained elevated throughout the treatment period. There was no correlation between IL-12 and IVEP parameters (p>0.05). However, RvE1 remained relatively high during the pre-treatment and decreased toward the end of treatment period (p<0.05). This in turn was associated with decline in some IVEP parameters (p<0.05).

Conclusion: Intra-ovarian administration of MSCs or ConM during repeated OPU did not enhance IVEP outcomes in Bos taurus heifers. The positive association between RvE1 and some of IVEP parameters could nominate RvE1 as a promising biomarker to predict IVEP parameters following repeated OPU.