Production of Egg Yolk Antibody (IgY) Against Vibrio Cholerae O1: Protective Effect  in Mice

Mohammad  Shoushtari; Ali Barat   Shooshtari; Sepideh  Asadi; Yousof  Karami; Mohsen  Honari; Ghorban Ali  Alizadeh; Mehdi  Zeinoddini; Javad  Fathi

doi:10.18502/ajmb.v15i4.13497

Mohammad Shoushtari Anatomical Sciences Research Center Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran
Ali Barat Shooshtari Karaj Branch, Islamic Azad University, Karaj, Iran
Sepideh Asadi Department of Microbiology and Immunology, University of Tehran, Faculty of Veterinary Medicine, Tehran, Iran
Yousof Karami Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Mohsen Honari Payame Noor University, Tehran, Iran
Ghorban Ali Alizadeh Payame Noor University, Tehran, Iran
Mehdi Zeinoddini Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran
Javad Fathi Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

DOI: https://doi.org/10.18502/ajmb.v15i4.13497

Keywords: Antibodies, Chicken, Immunoglobulin Y, Mice, Vibrio cholerae O1

Abstract

Background: Cholera is an acute intestinal infection caused by Vibrio cholera (V. cholera). The development of antibodies against specific V. cholerae may have a therapeutic effect. In the present research, we investigated the protective effect of egg yolk Immunoglobulin (IgY), which was produced by immunizing hens with formaldehyde-killed V. cholerae O1 and subsequently the isolated IgY was orally administrated to the V. cholerae O1 infected mice for evaluation of its immunizing capability.

Methods: In the current study, hens were immunized three times with formaldehyde-killed V. cholerae O1 (1.5×10⁷CFU/ml) and an equal volume of adjuvant. The IgY was isolated from egg yolk by polyethylene glycol method. The validity and activity of isolated IgY were confirmed with SDS-PAGE and ELISA methods, respectively. Subsequently IgY was orally administered to suckling mice following challenge with V. cholerae O1. ELISA results showed high antibody titer in the serum and egg yolk. Also, SDS-PAGE analysis showed successful purification of IgY and anti-V. cholerae IgY prevented the death of mice infected with V. cholerae O1. The anti-V. cholera IgY was administered at 2, 4, 6 hr intervals after 3 hr of inoculation of mice with V. cholerae O1.

Results: Results showed that the rate of surviving mice (2 mg/ml of IgY) were 60% after 4 hr and 40% after 6 hr and the rate of surviving mice (5 mg/ml of IgY) was 70% after 4 hr and 60% after 6 hr.

Conclusion: The findings suggested the egg yolk-driven IgY as a natural antibacterial protein, could be effective in the prevention and treatment of cholera disease.