Higher Improvement of Cardiac Function Following Myocardial Infarction using  Menstrual Blood Stromal/Stem Cells (MenSCs) Suspended in Conditioned Medium versus Conditioned Medium Alone in Rat Model

Mahmood  Manshori; Somaieh  Kazemnejad; Nasim  Naderi; Abolfazl  Shirazi; Maedeh  Arabian; Marzieh  Eghtedar Doost; Maryam  Darzi; Samaneh  Montazeri; Nahid  Aboutaleb; Hannaneh  Golshahi

doi:10.18502/ajmb.v15i3.12924

Mahmood Manshori Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Somaieh Kazemnejad Nanobiotechnology Research Center, Avicenna Research Institute, ACECR,Tehran, Iran
Nasim Naderi Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
Abolfazl Shirazi Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Maedeh Arabian Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
Marzieh Eghtedar Doost Nanobiotechnology Research Center, Avicenna Research Institute, ACECR,Tehran, Iran
Maryam Darzi Nanobiotechnology Research Center, Avicenna Research Institute, ACECR,Tehran, Iran
Samaneh Montazeri Nanobiotechnology Research Center, Avicenna Research Institute, ACECR,Tehran, Iran
Nahid Aboutaleb Physiology Research Center, Iran University of Medical Science, Tehran, Iran
Hannaneh Golshahi Nanobiotechnology Research Center, Avicenna Research Institute, ACECR,Tehran, Iran

DOI: https://doi.org/10.18502/ajmb.v15i3.12924

Keywords: Conditioned medium, Menstrual blood stem cells, Metaplasia, Mitochondrial transfer, Myocardial infarction

Abstract

Background: To evaluate the efficiency of Menstrual blood Stromal/Stem Cells (MenSCs) administration in Myocardial Infarction (MI), the effects of MenSCs and their derived conditioned Medium (CM) on cardiac function in MI rat model was assessed.

Methods: Animals were divided into four groups including sham group, MI group, MenSCs derived CM group (CM group), and MenSCs suspended in CM (MenSCs+ CM) group. The injection of different groups was carried out 30 min after ligation of left anterior descending coronary artery into the infarct border zone.

Results: The results showed a significant reduction in scar size after injection of MenSCs+CM compared to MI group. Ejection fraction and fractional shortening of MenSCs+CM group were higher than CM and MI group at day 28. Administration of MenSCs+CM led to much more survival of cardiomyocytes, and prevention of metaplastic development. Moreover, human mitochondrial transfer from MenSCs to cardiomyocytes was seen in group treated by MenSCs+CM. Indeed, MenSCs+CM treatment evoked nuclear factor-κB (NF-κB) down-regulation more than other treatments.

Conclusion: MenSCs+CM treatment could significantly ameliorate cardiac function by different mechanisms including inhibition of cartilaginous metaplasia, inhibition of NF-κB and mitochondrial transfer.