Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned  Media of Probiotics Bacteria

Fatemeh  Amini; Mohammad Bagher  Rezvani; Ronak Bakhtiari; Elham  Tabatabaei Ghomsheh

doi:10.18502/ajmb.v15i2.12017

Fatemeh Amini School of Dentistry, Shahed University of Medical Sciences, Tehran, Iran
Mohammad Bagher Rezvani Department of Restorative Dentistry, School of Dentistry, Shahed University, Tehran, Iran
Ronak Bakhtiari Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Elham Tabatabaei Ghomsheh Department of Restorative Dentistry, School of Dentistry, Shahed University, Tehran, Iran

DOI: https://doi.org/10.18502/ajmb.v15i2.12017

Keywords: Dental caries, Osteogenesis, Probiotics, Stem cells

Abstract

Background: Stem cells are used to treat numerous diseases; however, their lifespan is rather short. Factors such as probiotics affect and improve various cell lineage efficacies. The aim of this study was to investigate the effects of probiotics-conditioned media on dental pulp stem cell potentials in osteogenesis.

Methods: The experiment was initiated by culturing Lactobacillus casei and Lactobacillus acidophilus probiotics as well as DPS-7 cells. Bacterial supernatants were separated and concentrated as the conditioned media. The DPS-7 cells were treated with various concentrations of the conditioned media. Furthermore, MTT assay and alkaline phosphatase activity were used. The mRNA expression of three genes (bFGF, EGF-β and BMP-2) involved in osteogenesis was analyzed using a real-time polymerase chain reaction.

Results: The response of dental pulp stem cells to probiotics preconditioning promoted cell proliferation, increased alkaline phosphatase activity and upregulated bFGF and BMP-2 gene expression. Increased expression was significant for BMP-2 and moderate for bFGF; however, it was non-significant for EGF-β. The use of the two probiotics was the most effective.

Conclusion: In general, synergism of the combined probiotics preconditioning induces differentiation of DPS-7 cells into osteoblasts most effectively.