Proteome Analysis of Adult Acute Lymphoblastic Leukemia by Two-dimensional  Blue Native/Sodium Dodecyl Sulfate Gel Electrophoresis

Servin  Bagheralmoosavi; Parastou  Gholami; Mahdi  Amini; Mahdi  Alizadeh; Marjan  Yaghmaei; Sahar  Tavakkoli; Sina  Salari; Mahmood  Jeddi-Tehrani; Alireza  Ghasempour; Kambiz  Gilany; Mahdi  Shabani

doi:10.18502/ajmb.v15i1.11421

Servin Bagheralmoosavi Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Parastou Gholami Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mahdi Amini Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Mahdi Alizadeh Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Marjan Yaghmaei Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
Sahar Tavakkoli Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
Sina Salari HSCT Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mahmood Jeddi-Tehrani Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Alireza Ghasempour Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Kambiz Gilany Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
Mahdi Shabani Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ajmb.v15i1.11421

Keywords: Acute lymphoblastic leukemia, Biomarkers, Child, Mass, Matrix-assisted laser desorption-ionization, Molecular biology, Proteome, Spectrometry

Abstract

Background: Despite the significant progress in the treatment of Acute Lymphoblastic Leukemia (ALL) in children, it still remains as one of the most challenging malignancies in adults. Identification of new biomarkers may improve the management of adult ALL. Proteins expressed on the cell surface can be considered as disease-associated biomarkers with potential for diagnosis and targeted therapies. Thus, membrane proteome studies give essential information about the disease-related biomarkers.

Methods: We applied 2-dimensional blue-native SDS-PAGE technique followed by MALDI-TOF/TOF-mass spectrometry to study the cell membrane proteome of peripheral blood mononuclear cells of adult B-ALL patients in comparison to that of the healthy controls.

Results: Sixty seven differentially expressed protein spots were detected, among them 52 proteins were found to be up-regulated but the other 15 proteins were down-regulated in B-ALL. Five differentially expressed proteins, involved in energy metabolism pathways, were detected in B-ALL patients compared to the healthy control group.

Conclusion: Differentially expressed proteins provide an insight into the molecular biology of B-ALL. Further studies must be done to confirm our data to be considered as potential targets for detection and treatment of B-ALL.