Injectable Nano-Chitosan /CaCO3 (NCsC) Composite as Novel Pulpotomy Paste in Partially Pulpotomized Rabbit Incisors
Abstract
Purpose: Maintaining pulp vitality and functions are of paramount importance in different branches of modern dentistry like endodontic, conservative, pedodontics, and dental traumatology, the applying of tissue-engineering principles to create simple and effective scaffold material suitable for maintaining pulp vitality, represent a promising therapeutic option for treating immature and mature tooth with compromised pulp vitality.
To evaluate the pathophysiological response of the pulp to (nano-chitosan/CaCO3) scaffold as a potential novel capping material on traumatically exposed pulp of rabbit incisors.
Materials and Methods: Twenty-four upper incisors of twelve completely dentulous healthy New Zealand male rabbits were used, subdivided into two groups of 12 teeth. In each group, six teeth were used as a control group, where the pulp was traumatically exposed and partially amputated, left free of capping material, and the other six teeth, used as an experimental group, where the amputated pulp was capped with an injectable (nano-chitosan/CaCO3) composite. The cavities of both groups sealed with resin modified glass ionomer cement as the final restoration. Animals were sacrificed, and the teeth were collected for histological examination according to the sacrifice time (1 and 4 weeks).
Results: At 1 week, both groups showed a non-significant difference in inflammatory extent. Highly significant difference in calcific bridge formation (P=0.002), and dentin morphology (P=0.002). At a 4-week period, there is a non-significant difference in inflammatory response. High significant difference in dentine bridge formation (P=0.002). Non-significant difference in the dentin morphology (P=0.06). The nano-chitosan/CaCO3 group showed faster dentin bridge formation in both periods compared to the control group.
Conclusion: (nano-chitosan/CaCO3) composite is a promising novel pulpotomy material.
