Frequent Evaluation of Herpes Simplex Virus Type 2 in Women with Genital Herpes by Realtime PCR

Behnaz Montazeri; Haniyeh Bashi Zadeh Fakhar; Babak  Shaghaghi; Forouzan  Rostami

doi:10.18502/jmb.v12i3.16606

Behnaz Montazeri Islamic Azad University, Chalus Branch, Department of Medical Science, Chalous, Iran.
Haniyeh Bashi Zadeh Fakhar Department of Human Geneticse, Science and Research Branch, Branch, Islamic Azad University, Tehran, Iran.
Babak Shaghaghi Department of Laboratory Science, Chalous Branch, Islamic Azad University, Chalous, Iran.
Forouzan Rostami Department of Nursing, Faculty of Nursing and Midwifery, Islamic Azad University, Chalous Branch, Chalous,Iran.

DOI: https://doi.org/10.18502/jmb.v12i3.16606

Keywords: Herpes type 2, Genital herpes, Real time PCR.

Abstract

Background: Herpes simplex type 2 is a common infection worldwide. This disease is common in both developed and developing countries. Early detection of infection is very important to reduce the risk of infection. Real-time reliable PCR is a very sensitive and specific method that can be used as the best marker in determining the therapeutic effect by identifying a viral genome in an individual. The prevalence of herpes simplex virus type 2 in women with genital herpes was evaluated by Real time PCR method.

Methods: From January 1999 to March 2010, 45 samples of vaginal swabs and cervix of women with genital herpes were examined for HSV virus DNA detection using Real Time PCR.

Results: The mean age of the patients was 35.9 + 5.9. The percentage of positive cases of herpes simplex virus type 2 in the studied women was 22.2% and the history of infection with hpv was 33.3% vs. 12.5%. = 0.094 which was significant.

Conclusion: Clinical specimens of vaginal swabs from genital herpes caused by herpes simplex virus 2 can be quantitatively analyzed instead of nucleic acid extraction and amplification by PCR.