Diagnosis of Toxoplasma Infection in Allogenic Pre HCTSP  Patients Using Molecular Methods

Meysam  Yusefi; Zahra Arab-Mazar; Shirzad  Fallahi; Amirreza Javadi Mamaghani; Shahnaz  Sali; Naeem  Nikpour; Meisam  Barati; Arian  Karimi Rouzbahani; Farnaz  Kheirandish

doi:10.18502/ijpa.v17i2.9541

Meysam Yusefi Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Zahra Arab-Mazar Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shirzad Fallahi Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Amirreza Javadi Mamaghani Department of Parasitology and Mycology, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shahnaz Sali Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Naeem Nikpour Department of Hematology and Oncology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Meisam Barati Department of Cellular and Molecular Nutrition, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Arian Karimi Rouzbahani Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
Farnaz Kheirandish Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

DOI: https://doi.org/10.18502/ijpa.v17i2.9541

Keywords: Toxoplasma; Hematopoietic stem cell transplantation; Toxoplasmosis

Abstract

Background: We aimed to estimate the incidence of Toxoplasma infection in T. gondii-seropositive patients under allogeneic hematopoietic stem cell transplantation (HSCT).

Methods: The present research was a prospective study on 54 whole blood samples of allogeneic HSCT recipients, who were referring to bone narrow transplantation centers affiliated to Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2018. All patients were IgG positive against T. gondii.

Results: Overall, 54 Toxoplasma positive pre-HCTSP patients were enrolled. 53.7% (n= 29) were male, also 1.9% (n=1) had germ-line type of the disease. The Multiple myeloma patients had higher age in comparison with other disease, but pairwise comparison showed the difference of age between Multiple myeloma patients were statistically significant with Acute lymphoblastic leukemia, Acute myeloblastic leukemia and Huntington's disease (P< 0.05). The results of PCR assay showed 5.6% (n= 3) of the patients were infected with Toxoplasma.

Conclusion: PCR method has detected considerable incidence of Toxoplasma infection for monitoring HSCT recipients at risk for toxoplasmosis, and many patients who showed the incidence of toxoplasmosis had previous infections with the Toxoplasma parasite.