Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran

Morteza Ghanbari  Johkool; Aida  Vafae Eslahi; Milad  Badri; Elham  Hooshmand; Majid  Pirestani; Daniel  Jablonski; Rasool  Jafari; Abdolhossein  Dalimi

doi:10.18502/ijpa.v16i4.7882

Morteza Ghanbari Johkool Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
Aida Vafae Eslahi Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Milad Badri Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
Elham Hooshmand Department of Parasitology, Faculty of Veterinary Medicine, Rasht Branch, Islamic Azad University, Guilan
Majid Pirestani Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Daniel Jablonski Department of Zoology, Comenius University in Bratislava, Mlynská dolina, Ilkovičova 6, 842 15 Bratislava, Slovakia
Rasool Jafari Department of Medical Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Abdolhossein Dalimi Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

DOI: https://doi.org/10.18502/ijpa.v16i4.7882

Keywords: Abbreviata kazakhstani-ca; Molecular identifi-cation; Pseudopus apodus; Physalopteridea; Iran

Abstract

Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A. kazakhstanica (Spirurida: Physalopteridea) in Pseudopus apodus in Iran.

Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-2018. The nematodes were isolated from stomach. After morphological study, the genomic DNA of the parasites was extracted using CTAB method. The DNA was used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega.

Results: After detailed microscopic examination, the A. kazakhstanica was identified. The cox1 sequences confirmed the species of helminth. The new sequences of A. kazakhstanica were submitted to GenBank under the accession number MK578751-2.

Conclusion: Regarding the limited data on parasitological status of Iranian reptiles, more specific and comprehensive investigations are needed to identify the parasitic fauna.