Leukocyte Behavior in Mesenteric Microcirculation upon Exper-imental By Leishmania Spp. in BALB/c Mice

Herintha Coeto  Neitzke-Abreu; Rhuan Carlos Souza  Caetano; Kárin Rosi  Reinhold-Castro; Manoel Sebastião da  Costa Lima-Junior; Wagner José Tenório dos  Santos; Gessilda de Alcantara  Nogueira de Melo; Roberto Kenji Nakamura  Cuman; Sandra Mara  Alessi Aristides; Thais Gomes  Verzignassi Silveira; Maria Valdrinez Campana Lonardoni

doi:10.18502/ijpa.v16i4.7869

Herintha Coeto Neitzke-Abreu Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil
Rhuan Carlos Souza Caetano Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil
Kárin Rosi Reinhold-Castro Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil
Manoel Sebastião da Costa Lima-Junior Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE, Brazil
Wagner José Tenório dos Santos Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, PE, Brazil
Gessilda de Alcantara Nogueira de Melo Departament of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, PR, Brazil
Roberto Kenji Nakamura Cuman Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil
Sandra Mara Alessi Aristides Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil
Thais Gomes Verzignassi Silveira Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil
Maria Valdrinez Campana Lonardoni Postgraduate Program of Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil

DOI: https://doi.org/10.18502/ijpa.v16i4.7869

Keywords: Leishmania; Rolling; Cellular adhesion; Leukocyte

Abstract

Background: We aimed to determine the cellular recruitment (leukocyte rolling and adhesion) by which the Leishmania (Viannia) braziliensis, L. (Leishmania) amazonensis, and L. (Leishmania) major species in the mesenteric microcirculation of BALB/c mice.

Methods: Five experimental groups were considered: group 1 (L. braziliensis); group 2 (L. amazonensis); group 3 (L. major); group 4 (control group with PBS); group 5 (negative control group), analyzed 3, 6, 12, and 24 h after parasite inoculation.

Results: Infections by the different Leishmania species caused an increase in the number of rolling leukocytes: L. braziliensis a peak at 6 h; L. amazonensis and L. major a peak at 3 h. The Leishmania infections induced leukocyte adhesion: L. major and L. amazonensis showed an increase after 3 and 6 h, respectively.

Conclusion: The kinetics of cellular recruitment in Leishmania infections, leading to infection susceptibility or resistance, indicates that distinct mechanisms regulate the initial response to Leishmania infection and determine its course.