Molecular Identification of Nematodes (Superfamily: Strongylida) Traced in Herbivores Excrement Found in Wildlife from Western Iran

Mohammad Reza  Vafaei; Mohammad  Safaie; Elham  Kazemirad; Hamed  Mirjalali; Zaynab  Askari; Mehdi  Mohebali; Gholamreza  Mowlavi

doi:10.18502/ijpa.v20i4.20465

Mohammad Reza Vafaei Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Safaie Provincial Department of Environment, Kermanshah, Iran
Elham Kazemirad Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Hamed Mirjalali Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Scienc-es, Tehran, Iran
Zaynab Askari Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mehdi Mohebali Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Gholamreza Mowlavi Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijpa.v20i4.20465

Keywords: Strongylida; Western Iran; Herbivores; Parasitology

Abstract

Background: In wildlife, the identification of parasitic infections should be pursued seriously in countries facing endangered species of animals in their geographical territories. We aimed to increase understanding of the possible role of wildlife herbivore reservoirs in the emergence of helminth infections in Kermanshah Province, western Iran.

Methods: Sixty-five feces from Gazelle (Gazella subgutturosa) (N = 36 samples) and wild goat (Capra aegagrus) (N = 14 samples) were investigated. The samples were microscopically examined for gastrointestinal helminth eggs, and genomic DNA was extracted from the identified eggs. The internal transcribed spacer 2 (ITS2) region of ribosomal DNA was amplified and sequenced. Phylogenetic analysis of the nucleotide sequences confirmed the species identity.

Results: The most common species circulating in the hosts were Teladorsagia circumcincta, Marshallagia spp., and Nematodirus oiratianus, all of which are reported in the wildlife in western Iran for the first time.

Conclusion: These findings emphasize the importance of continuously assessing the parasite status of wildlife and similar routine surveillance in domestic environments to detect and manage potential zoonotic parasite species.