Molecular Genotyping of Giardia duodenalis in Humans in the Yazd County, Central of Iran

Elaheh  Hezardastan; Ali Fattahi  Bafghi; Gilda  Eslami; Mahmoud  Vakili

doi:10.18502/ijpa.v19i1.15216

Elaheh Hezardastan Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Ali Fattahi Bafghi Department of Parasitology and Mycology, Infectious Diseases Research Center, School of Medicine, Shahid Sadoughi Uni-versity of Medical Sciences, Yazd, Iran
Gilda Eslami Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mahmoud Vakili Health Monitoring Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

DOI: https://doi.org/10.18502/ijpa.v19i1.15216

Keywords: Giardia duodenalis; Genotyping; Assemblages; Iran

Abstract

Background: We aimed to investigate the molecular genotyping of Giardia duodenalis in Humans in the Yazd County, Central, Iran.

Methods: Total of 35 fecal samples were collected from patients referred to Yazd Central Laboratory, Yazd, Iran from February to July 2022. All the samples were included in this study after microscopic observation of G. duodenalis. DNA samples were extracted using related kit and were analyzed by Nano Drop. The molecular assessment was carried out using semi-nested PCR using the target gene of gdh. All amplified samples were sequenced using Sanger method. BLAST analyzed the sequences for assemblage identification.

Results: Out of 35 samples, 24 (68.57%) and 11 (31.43%) were male and female, respectively. All included samples were amplified using the specific gdh primer pair. The molecular analysis showed 17 isolates (48.57%) as assemblage BIV, 8 isolates (22.86%) as assemblage BIII, 6 isolates (17.14%) as assemblage AII and 4 isolates (11.43%) as assemblage AIII (P<0.05).

Conclusion: Assemblages A and B are the most prevalent in Central Iran. The molecular identification of G. duodenalis isolates from animals and implementing control programs