Transcriptome Profiling of Male Adult Angiostrongylus cantonensis

  • Guo Yue School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Yu Liang Emergency Department, South Taihu Hospital, Gangnan Road 1566, Huzhou, Zhejiang, China
  • Dong Ying School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Zhou Hongchang School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Shang Ya Qian School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Zhang De Long School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Chen Gao Qiang School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Jiang Bei School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Wu Wan Ting School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China
  • Lu Mingke School of Life Sciences, Xiamen University, Fujian, China
Keywords: Angiostrongylus cantonen-sis; Helminths; Transcriptome

Abstract

Background: The pathogen of angiostrongyliasis is the parasite Angiostrongylus cantonensis, and the transcriptome profiling of the male adult was unclear. We aimed to understand how the male adults adapt, so the expression profile of A. cantonensis adult males was analyzed.

Methods: In order to improve the understanding of the transcriptome of adult males, RNA from three groups of male adult A. cantonensis was extracted and reverse transcribed to construct cDNA libraries. After sequencing, annotation of unigenes and transcripts was performed by querying the NR (Non-Redundant Protein Sequence Database), GO (Gene Ontology) and COG/ KOG (Clusters of Orthologous Groups of proteins/euKaryotic Ortholog Groups) databases.

Results: For each group of adults, 43,260,894 raw reads and 43,200,341 clean reads were obtained. After successful assembly, 87,649 unigenes and 146,895 transcripts were obtained. Annotation of the unigenes and transcripts was identical and male adults expressed a series of genes encoding proteins specific to the male gender at the adult stage, such as proteins involved in energy metabolism, energy synthesis and transport. Expression of the ribosome pathway suggests a relationship with the physical activities during the adult male stage.

Conclusion: The transcriptome analysis is a good reference to understand further the expression profile of male adult A. cantonensis.

Published
2023-10-07
Section
Articles