Preliminary Information of Iranian Lizard Leishmania Pro-mastigote Transcriptome Sequencing by Next -Generation Sequencing (NGS) Method

Farveh  Ehya; Sima  Kalantari; Mojgan  Bandehpour; Bahram  Kazemi

doi:10.18502/ijpa.v18i3.13759

Farveh Ehya Department of Biotechnology, Tarbiat Modares University, Tehran, Iran
Sima Kalantari Digestive Oncology Research Center, Digestive Diseases Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Teh-ran, Iran
Mojgan Bandehpour Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Bahram Kazemi Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/ijpa.v18i3.13759

Keywords: Lizard leishmania; RNA sequencing; Transcriptome; Next generating sequencing

Abstract

Background: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far.

Methods: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. Leishmania promastigotes were cultured in RPMI₁₆₄₀ culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China).

Results: The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania.

Conclusion: Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system.