Transcriptome Profiling and Bioinformatic Analysis of the Fourth-Stage Larvae of Angiostrongylus cantonensis

Yue  Guo; Hui  Zhang; Haiyan  Dong; Ming Ke  Lu; Xiaoyu  Li

doi:10.18502/ijpa.v17i4.11287

Yue Guo School of Medicine, Huzhou University, Huzhou, Zhejiang, China
Hui Zhang School of Medicine, Huzhou University, Huzhou, Zhejiang, China
Haiyan Dong School of Medicine, Huzhou University, Huzhou, Zhejiang, China
Ming Ke Lu School of Life Sciences, Xiamen University, Fujian, China
Xiaoyu Li School of Medicine, Huzhou University, Huzhou, Zhejiang, China

DOI: https://doi.org/10.18502/ijpa.v17i4.11287

Keywords: Angiostrongylus cantonensis; Fourth-stage larvae; Transcriptome; Next-generation sequencing

Abstract

Background: To explore the transcriptome profiling of the fourth-stage larvae of Angiostrongylus cantonensis.

Methods: Two groups of fourth-stage larvae were collected to extract total RNA in Zhejiang, China 2020. Then, mRNA was separated and reverse transcribed into cDNA. Next-generation sequencing was used to explore the transcriptome information. Finally, to obtain the biological annotation information, the transcriptome information was run against the related databases, including Nr, GO, COG, KOG and ORF.

Results: Overall, 128667 unigenes and 193059 transcripts were obtained. The Nr annotations of unigenes and transcripts showed that A. cantonensis was the 5th and 4th most related species, respectively. Meanwhile, the annotation of unigenes and transcripts by querying GO, COG, KOG and ORF showed that L4 was extremely active in gene expression, concerning signal transduction, transcription, posttranslational modification, metabolism, etc.

Conclusion: The fourth-stage larvae of A. cantonensis have their own profiling in the transcriptome, which is related to signal transduction, transcription, posttranslational modification, metabolism, etc.