Serological Screening of Patients Diagnosed with Alveolar  Echinococcus Disease in Their Home Regions

Onder  Akkas; Hakan   Uslu; Isa  Yilmaz; Yener  Aydın; Ercan  Korkut; Ahmet   Yilmaz

doi:10.18502/ijpa.v17i4.11283

Onder Akkas Department of Medical Microbiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey
Hakan Uslu Department of Medical Microbiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
Isa Yilmaz Department of Animal Production and Technology, Faculty of Applied Sciences, Mus Alparslan University, Mus, Turkey
Yener Aydın Department of Thoracic Surgery, Faculty of Medıcıne, Ataturk University, Erzurum, Turkey
Ercan Korkut Department of General Surgery, Faculty of Medıcıne, Atatürk University, Erzurum, Turkey
Ahmet Yilmaz Department of Medical Laboratory Techniques, Erzurum Vocational School of Health, Ataturk University, Erzurum, Turkey

DOI: https://doi.org/10.18502/ijpa.v17i4.11283

Keywords: Echinococcosis; Echinococcus multilocularis; Western blot; Echinococcus alveolaris

Abstract

Background: We aimed to determine the prevalence of Alveolar echinococcusis using ELISA in our region, to perform the specific differentiation of species by using the Western Blott method, to diagnose and treat this disease effectively in early periods, and to inform the patients about the results quickly.

Methods: From the provinces of Erzurum, Kars, Ardahan, Iğdır, Ağrı and Erzincan in the Eastern Anatolia Region, Turkey in August-September 2017, blood samples were provided from 305 participants (volunteer patients and their relatives), including 151 females (49.50%), whose ages ranged between 6-85 yr and were diagnosed as Alveolar echinococcosis (AE) and operated in concerned clinics.

Results: EIgG ELISA was determined as positive in 29 (9.5%) participants, including 17 (11.3%) females and 12 (7.8%) males. In addition, Em2-Em18 ELISA was also determined as positive in 15 (4.9%) participants, including 9 (6%) females and 6 (3.9%) males. Tweleve (3.94 %) of these findings were observed as positive in terms of both tests. Through the verification done by Anti-EWB IgG, Echinococcus multilocularis (EM) was identified in 7 (21.9%) of the female participants, E. granulosus (EG) in 1 (3.1%), and both parasites in 2 (6.2%). For male participants, EM was determined in 3 (9.3%) of them, EG in 1 (3.1%), and both parasites in 1 (3.1%).

Conclusion: AE continues to threaten public health in the region. In families where AE is detected positive, the reason for disease is based on nutrition; thus, it will be proper to check up all family members in the terms of infection.