Transcriptomic Profiles of MV4-11 and Kasumi 1 Acute Myeloid Leukemia Cell Lines Modulated by Epigenetic Modifiers Trichostatin A and 5-Azacytidine

Mat Jusoh Siti Asmaa; Hamid Ali  Al-Jamal; Abdul Rahim Hussein; Badrul Hisham Yahaya; Azlan Husin; Roslin Hassan; Faezahtul Arbaeyah Hussain; Shaharum Shamsuddin; Muhammad Farid  Johan

doi:10.18502/ijhoscr.v14i1.2362

Mat Jusoh Siti Asmaa
Hamid Ali Al-Jamal
Abdul Rahim Hussein
Badrul Hisham Yahaya
Azlan Husin
Roslin Hassan
Faezahtul Arbaeyah Hussain
Shaharum Shamsuddin
Muhammad Farid Johan

DOI: https://doi.org/10.18502/ijhoscr.v14i1.2362

Keywords: Acute myeloid leukemia; Epigenetics, Histone deacetylase inhibitors; 5-Azacytidine; Gene expression

Abstract

Background: Acute myeloid leukemia (AML) is the most common form of acute leukemias in adults which is clinically and molecularly heterogeneous. Several risk and genetic factors have been widely investigated to characterize AML. However, the concomitant epigenetic factors in controlling the gene expression lead to AML transformation was not fully understood. This study was aimed to identify epigenetically regulated genes in AML cell lines induced by epigenetic modulating agents, Trichostatin A (TSA) and 5-Azacytidine (5-Aza).

Materials and Methods: MV4-11 and Kasumi 1 were treated with TSA and/or 5-Aza at IC₅₀ concentration. Gene expression profiling by microarray was utilized using SurePrint G3 Human Gene Expression v3. Gene ontology and KEGG pathway annotations were analyzed by DAVID bioinformatics software using EASE enrichment score. mRNA expression of the differentially expressed genes were verified by quantitative real time PCR.

Results: Gene expression analysis revealed a significant changes in the expression of 24,822, 15,720, 15,654 genes in MV4-11 and 12,598, 8828, 18,026 genes in Kasumi 1, in response to TSA, 5-Aza and combination treatments, respectively, compared to non-treated (p<0.05). 7 genes (SOCS3, TUBA1C, CCNA1, MAP3K6, PTPRC, STAT6 and RUNX1) and 4 genes (ANGPTL4, TUBB2A, ADAM12 and PTPN6) shown to be predominantly expressed in MV4-11 and Kasumi 1, respectively (EASE<0.1). The analysis also revealed phagosome pathway commonly activated in both cell lines.

Conclusion: Our data showed a distinct optimal biological characteristic and pathway in different types of leukemic cell lines. These finding may help in the identification of cell-specific epigenetic biomarker in the pathogenesis of AML.