Different Immune Reconstitution between Cord Blood and unrelated Bone Marrow Transplantation with Relation to Chronic Graft-versus-Host Disease

Hitoshi   Yoshida; Midori  Koike; Midori   Koike; Keiichi Nakata; Akihisa Hino; Shigeo  Fuji; Hiroaki Masaie; Chihiro  Oka; Akemi Higeno; Atsushi Idota; Tomoyuki  Yamasaki; Jun Ishikawa

doi:10.18502/ijhoscr.v14i1.2323

Hitoshi Yoshida
Midori Koike
Midori Koike
Keiichi Nakata
Akihisa Hino
Shigeo Fuji
Hiroaki Masaie
Chihiro Oka
Akemi Higeno
Atsushi Idota
Tomoyuki Yamasaki
Jun Ishikawa

DOI: https://doi.org/10.18502/ijhoscr.v14i1.2323

Keywords: Immune reconstitution; Cord blood transplantation; Unrelated bone marrow transplantation; Chronic GVHD

Abstract

Background: Advances of allogeneic hematopoietic cell transplantation (allo-HCT) have brought long-term survival to the patients with hematologic malignancies. Chronic graft-versus-host disease (GVHD) is one of major problems for the long- term survivors after allo-HCT. Dysregulation of immune reconstitution has been reported to be involved in the pathogenesis of chronic GVHD. Differences of immune reconstitution between cord blood transplantation (CBT) and unrelated bone marrow transplantation (uBMT) remain unclear in long-term survivors. We investigated immune reconstitution in patients surviving for more than 2 years after CBT (n=21) or uBMT (n=20) without relapse of underlying disease.

Materials and Methods: Using flow cytometric analysis of peripheral blood, we investigated immune reconstitution of T cells, B cells, and NK cells between CBT and uBMT patients. We collected clinical data regarding allo-HCT and examined the relation of immune reconstitution to the development of chronic GVHD.

Results: Between CBT and uBMT patients, we found significant differences in absolute cell number of CD8+ as well as CD19+ cell and CD4/CD8 ratio even more than 2 years after allo-HCT. Among uBMT patients, absolute cell number of naive CD4+ cell was significantly lower in patients with chronic GVHD. In addition, we found significant differences in absolute cell number of CD19+ cell, especially naive B cell between patients with and without chronic GVHD in both CBT and uBMT patients.

Conclusion: These results suggest that differences of immune recovery between CBT and uBMT patients may exist even in patients surviving for more than 2 years and might be related to the development of chronic GVHD.