Evaluation of the Role of Tumor-Infiltrating Lymphocytes and CD8+ Cytotoxic Lymphocytes in the Survival of Patients with Breast Cancer

Shahram Shojaei; Mozaffar Aznab; Amirmasoud Rahimi; Seyed Mojtaba  Ahmadi; Kumars Eslampia; Arash Golpazir; Masoud Iravani

doi:10.18502/ijhoscr.v17i1.11708

Shahram Shojaei Bistoon Hospital, Kermanshah, Iran
Mozaffar Aznab Department of Internal Medicine, Talaghani Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
Amirmasoud Rahimi Kermanshah University of Medical Sciences, Kermanshah, Iran
Seyed Mojtaba Ahmadi Department of Clinical Psychology School of Medicine, Kermanshah University of Medical Science, Kermanshah, Iran
Kumars Eslampia Department of Surgery, Bistoon Hospital, Kermanshah, Iran
Arash Golpazir Department of Surgery, Cancer Surgery, Kermanshah University of Medical Sciences, Kermanshah, Iran
Masoud Iravani Masoud Gastroenterology and Liver Clinic, Tehran, Iran

DOI: https://doi.org/10.18502/ijhoscr.v17i1.11708

Keywords: Breast cancer; CD8 cytotoxic lymphocytes; Overall Survival; Tumor-infiltrating lymphocytes

Abstract

Background: This study aimed to evaluate the significance of tumor lymphocyte infiltration (TIL) and the number of CD8+ T cells in breast cancer and their relationship with the other clinicopathological factors and overall survival (OS) was investigated.

Materials and Methods: The studied samples were breast cancer patients (2005-2017) referring to the medical oncology departments for treatment. Pathologic samples of breast cancer patients were evaluated in terms of TIL and positive immunohistochemical staining for CD8 cytotoxic cells.

Results: 299 patients were entered into the study, 3 male and 296 female. Their mean follow-up period was 61 months. Statistical findings indicated that lymph involvement is more accompanied by low TIL within the tumor (0.011). Correlations were observed between the estrogen, progesterone receptors, P53 state, and TIL; which were significant by P-value<0.049, P-value=0.024, P-value =0.002, respectively. With any Ki67 value, the number of patients with less than 30% TIL was more considerable than the two other groups with lymphocyte cut-off of 30-50% and more than 50%. Comparison of the OS of patients with positive and negative CD8 cytotoxic lymphocytes in 45 patients with lymphocyte infiltration of equal or more than 40% showed that the OS results were in favor of patients with CD8+ cytotoxic lymphocyte (0.022). Out of 299 patients, 17 died.

Conclusion: Our findings showed that in cases of CD8+ cytotoxic lymphocytes in tumors, the OS of the patients will be enhanced which can act as an independent.