Diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with cerebral venous thrombosis

Zoofa  Zayani; Etrat  Hooshmandi; Afshin  Borhani-Haghighi; Mousa  Rahimi; Vahid Reza  Ostovan; Nima  Fadakar; Reza  Tabrizi; Mahnaz  Bayat; Seyedeh Shamim  Hojati; Negin  Gharbi; Hamideh  Mahmoudinasab

doi:10.18502/cjn.v23i2.16840

Zoofa Zayani Department of Biology, Islamic Azad University, Zarghan Branch, Zarghan, Iran
Etrat Hooshmandi Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Afshin Borhani-Haghighi Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mousa Rahimi Laboratory of Basic Sciences, Mohammad Rasul Allah Research Tower, Shiraz University of Medical Sciences, Shiraz, Iran
Vahid Reza Ostovan Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Nima Fadakar Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Reza Tabrizi Noncmmunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
Mahnaz Bayat Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Seyedeh Shamim Hojati Department of Biology, Islamic Azad University, Zarghan Branch, Zarghan, Iran
Negin Gharbi Department of Biology, Islamic Azad University, Zarghan Branch, Zarghan, Iran
Hamideh Mahmoudinasab Department of Biology, Islamic Azad University, Zarghan Branch, Zarghan, Iran

DOI: https://doi.org/10.18502/cjn.v23i2.16840

Keywords: Venous Thrombosis; Stroke; Long Non-Coding RNA; Myocardial Infarction Associated Transcript; Gene Expression

Abstract

Background: Long non-coding ribonucleic acids (lncRNAs) have been implicated as possible circulating stroke indicators. This study focused on the expression status of antisense non-coding ribonucleic acid in the INK4 locus (ANRIL) and myocardial infarction associated transcript (MIAT) in patients with cerebral venous thrombosis (CVT).

Methods: In this study, fifty patients with CVT and one hundred age/gender-matched individuals as controls were included. The circulating levels of ANRIL and MIAT in the first 24 hours after admission were evaluated using the quantitative real-time polymerase chain reaction (RT-PCR) method. We compared the expression levels of ANRIL and MIAT between patients and controls using the independent two-sample t-test. Subgroup analysis was used to investigate the association of lncRNAs with clinical characteristics in patients with CVT. Receiver operating characteristic (ROC) curve analyses were conducted to evaluate the diagnostic value of two lncRNAs in patient assessment.

Results: The relative expression of lncRNAs ANRIL and MIAT significantly decreased in patients compared to the control. ANRIL and MIAT were shown as potential markers for discriminating patients with CVT from the healthy controls with an area under the curve (AUC) of 0.98 and 0.99, respectively.

Conclusion: For the first time, we found down-regulation and diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with CVT.