Association Between Expression of Interleukin-32 Gene and Various Helicobacter pylori Virulence Factors in Human Infected Gastric Biopsy

Abstract

Helicobacter pylori (H. pylori) is a spiral bacterium that infects the human gastric mucosa. Various clinical aspects of the infection may mirror distinctive forms of cytokine expression. It correlates with immune cell penetration to the gastric mucosa with numerous cytokines production and gastric inflammation. IL-1 and IL-8 directly contribute to H. pylori effected gastritis. IL-32 is a pro-inflammatory cytokine categorized by the training of Immune cell activation, which has a vital role in human immunity. H. pylori virulence and danger factors are critical in gastritis, such as the outer inflammatory protein (OipA) and the cytotoxin associated gene a (cagA). We aimed to study the IL-32 mRNA expression in H. pylori-positive and negative patients as well as its relation with bacterial cagA, oipA, and severity of gastritis. Endoscopic biopsies were taken from the antrum of 60 H. pylori-infected patients and 62 uninfected individuals. Mucosal IL-32 mRNA expression was assessed by real-time PCR. With PCR, the H. pylori virulence factors were evaluated. Showed that the mRNA expression of IL-32 levels was significantly lower in biopsies of H. pylori-uninfected patients compared to positive individuals (P=0.01). A straight communication between virulence factor up, cagA, and heightening in IL-32 mRNA expression (P<0.001) was observed. Furthermore. IL-32 mRNA expression levels were approximately equal in both chronic and active gastritis (P=0.1). IL-32 may have a critical role in different situations like inflammation and the severity of inflammatory changes in the gastric mucosa.