Correlation Between I/T Ratio, S100B, Interleukin-10 Serum, and NEWS2 Score in Sepsis

Novianti Anggie  Lestari; Lisyani  Budipradigda Suromo; Meita Hendrianingtyas; Ariosta Setyadi; Dwi Retnoningrum

doi:10.18502/acta.v61i10.15658

Novianti Anggie Lestari Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
Lisyani Budipradigda Suromo Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
Meita Hendrianingtyas Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
Ariosta Setyadi Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
Dwi Retnoningrum Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

DOI: https://doi.org/10.18502/acta.v61i10.15658

Keywords: I/T ratio; S100B; Interleukin-10; National early warning score (NEWS2); Sepsis

Abstract

Immature/total neutrophils (I/T) ratio is a simple and inexpensive method used as an early sign of bacterial infection. Meanwhile, the S100B protein is found in glial cells and functions as a neuroprotection. Then, the interleukin-10 cytokine acts as an anti-inflammatory cytokine during infection. In addition, the NEWS2 score is used to see the early and fast changes in patients with sepsis. The purpose of the study was to prove the correlation between the I/T ratio, S100B, IL-10, and NEWS2 score in patients with sepsis. A cross-sectional study was conducted on 34 patients with sepsis from September 2020 to February 2021. I/T ratio was calculated in 100 leukocytes from microscopic blood smear preparation. Levels of S100B and IL-10 serum were measured using the ELISA method. Pearson correlation test was used for normally distributed data, and the Spearman correlation test was utilized for abnormally distributed data. Correlation test between I/T ratio, S100B, and IL-10 with NEWS2 score respectively showed values of r=0.58; P=0.01, r=0.36; P=0.03, and r=0.39; P=0.02, in which P<0.05. There was a moderate positive correlation between I/T ratio and NEWS2 score, a weak positive correlation between S100B and NEWS2 score, and a weak positive correlation between IL-10 and NEWS2 score.