Effect of Extracellular Vesicles Derived From Peripheral Blood Mononuclear Cells on K562 Leukemia Cell Line

Hajar Mardani  Valandani; Roohollah Mirzaee Khalilabadi; Amir Mohammad Zahedi

doi:10.18502/acta.v61i8.14901

Hajar Mardani Valandani Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran
Roohollah Mirzaee Khalilabadi Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran
Amir Mohammad Zahedi Department of Physiotherapy, School of Rehabilitation, Tehran University of Medical Sciences, Tehran, Iran.

DOI: https://doi.org/10.18502/acta.v61i8.14901

Keywords: Chronic myelogeneous leukaemia; k562 cell; Extracellular vesicles; Peripheral blood mononuclear cells

Abstract

Recently, extracellular vesicles (EVs) are generating considerable interest in terms of their ability. EVs defined themselves as a route for intercellular communication between the origin cells and the recipient ones by transferring information. This paper investigates the influence of peripheral blood mononuclear cells derived EVs on proliferation and apoptosis of the chronic myelogenous leukemia cell line k562. Trypan blue staining was used to detect cell viability subsequently, metabolic activity was assessed by the MTT assay. Cell apoptosis and cell cycle progression were evaluated using flowcytometric assay after treatment of k562 cell line with MNC derived EVs. Our results showed that MNC-EVs have no inhibitory effect on k562 cell growth and proliferation. Our data did not reveal any significant variations in the case of enhancing k562 cell line growth following treatment with MNC derived EVs. It seems tumour-derived extracellular vesicles support tumour cells growth by communicating with each other through their extracellular vesicles.