Investigation of Metallo-Beta-Lactamase Genes (L1 and NDM1) in Isolates of Stenotrophomonas maltophilia

Rabeeh Izadi Amoli; Jamileh Nowroozi

doi:10.18502/acta.v60i7.10215

Rabeeh Izadi Amoli Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
Jamileh Nowroozi Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran

DOI: https://doi.org/10.18502/acta.v60i7.10215

Keywords: Stenotrophomonas maltophilia; Antibiotic; L1 metallo-beta-lactamase gene; New Delhi metallo-beta-lactamase 1 gene

Abstract

Stenotrophomonas maltophilia is resistant to a wide range of antibiotics. This study aimed to investigate the beta-lactamase, ESBL, and MBL enzymes and also L1and NDM1 genes in isolates of Stenotrophomonas maltophilia. Antibiotic susceptibility test, beta-lactamase, ESBL, and MBL test were done on 23 isolated Stenotrophomonas maltophilia. The presence of L1 and NDM1 genes was investigated on isolated MBL bacteria by the PCR method. The results showed the most effective antibiotics were tigecycline and gentamicin (100%), and the highest resistance was observed with aztreonam (56.22%). 39.13% of isolates were ESBL, 82.66% of isolates were beta-lactamase positive, and 60.86% of isolates were Metallo-beta-lactamase positive. 39.13% of MBL-positive isolates were positive for the L1 gene, but the NDM1 gene was not seen. Results of this study showed higher resistance to beta-lactam antibiotics also the presence of the l1 gene. ESBL and MBL producing S. maltophilia are frequently resistant to a wide range of antibiotics. Therefore, needs to be done antibiotics sensitivity tests, ESBL, and MBL tests before treatment for this bacterium.