Evaluation of Antibacterial and Anti-Fungal Effect of Zinc Oxide Nano Particles to Complete Denture Tissue Conditioners

Amin Khaleghi; Parisa Faghani; Mojtaba  Azarian Borujeni

doi:10.18502/ijds.v17i3.7533

Amin Khaleghi Post Graduated Student, Department of Orthodontics, Student Research Committee, Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Parisa Faghani Dentist, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
Mojtaba Azarian Borujeni Assistant Professor, Department of Prosthodontics, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.

DOI: https://doi.org/10.18502/ijds.v17i3.7533

Keywords: Zinc oxide, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis.

Abstract

Introduction: The tissue conditioners are used to treat and prepare denture supporting tissue. This study aimedto evaluate the antibacterial and antifungal effect of zinc oxide nanoparticles on total denture tissue conditioners.

Materials & Methods: In this laboratory study, 144 samples were collected and evaluated. Synthesized ZnO nanoparticles usingthe optic-optic method and, they were homogeneous with a mass fraction (0.625, 1.25, 2.5, 5, 10, and 20) according to the principles of the MIC method with tissue concentrators. In the present study, four bacterial species (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis), which represent different types of pathogen microorganisms, were used. For measuring the growth rate of microorganisms, a spectrophotometer was used based on turbidity with donereadings at 600 nm. Included tests were ANOVA and Tukey post hoc tests. Considered significant level of 0.05.

Results: In the present study, increasing ZnO nanoparticles to tissue concentrators reduced the growth of all microorganisms studied. In the concentration of 20% absolute growth inhibition for Candida albicans, Streptococcus mutans, Enterococcus faecalis and Pseudomonas aeruginosa occurred. The best concentration for Candida, Enterococcus faecalis is in the concentration of 20% ZnO and, for Streptococcus mutans, Pseudomonas aeruginosa at a concentration of 5% ZnO.

Conclusion: Increasing the ZnO nanoparticles to improve tissue improves the growth of microorganisms.