The Effect of Photodynamic Therapy with Photosan and Different Concentrations of Methylene Blue on Candida Albicans had been Grown on Denture

Ali Salehi Mazandarani; Shahram Amini; Amir Mansour Shirani; Mones  Sanjaripour

doi:10.18502/ijds.v18i3.11239

Ali Salehi Mazandarani Graduated of Dentistry, School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.
Shahram Amini Assistant Professor, Department of Periodontology, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Amir Mansour Shirani Assistant Professor, Department of Oral Medicine, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Mones Sanjaripour Postgraduate Student, Department of Oral Medicine, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

DOI: https://doi.org/10.18502/ijds.v18i3.11239

Keywords: Photochemotherapy, Methylene blue, Candida albicans, Dentures.

Abstract

Introduction: In some studies, photodynamic therapy with some light sources and light-absorbing materials were used against Candida. In this study, the antifungal effects of 630 nm photosan with different concentrations of methylene blue on Candida albicans that had been grown on dentures was investigated. In data searches no similar study was found.

Materials & Methods: This experimental laboratory study was performed in Isfahan Azad University in 2019-2020. On total 26 similar dentures were prepared. Suspension of 0.5 McFarland Candidia albicans was inoculated on the dentures. The samples were put into three groups (seven in each) with different concentrations of methylene blue (0.1, 0.01, 0.001 g/l) and photosan with a wavelength of 630 nm was used. Five control samples including untreated control, photosan alone and three controls with methylene blue concentrations only were considered. The number of colonies was counted using manual and ocular methods. Data were analyzed with T-test, ANOVA.

Results: The mean number of Candida albicans grown on dentures after photodynamic therapy and different concentrations of methylene blue was significantly different from control groups (p value < 0.05). The mean number of Candida albicans grown on dentures after photodynamic therapy were significantly different between the three concentrations of methylene blue (p value < 0.001). Methylene blue with a concentration of 0.1 mg had significantly better efficacy against candida compared with two other concentrations (p value < 0.001).

Conclusion: Photodynamic therapy with photosan and methylene blue with a concentration of 0.1 provided better results compared to others in reducing Candida albicans.