In Vitro Efficacy of Tricalcium Phosphate and Casein Phosphopeptide Amorphous Calcium Phosphate Fluoride for Remineralization of Enamel White Spot Lesions

Alireza  Haerian; Soghra  Yasaei; Elaheh  Rafiei; Seyed Vahid  Malek Hosseini; Negin  Karimi

doi:10.18502/fid.v21i33.16436

Alireza Haerian Department of Orthodontics, Faculty of Dentistry,Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada
Soghra Yasaei Department of Orthodontics, Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Elaheh Rafiei Department of Orthodontics, Faculty of Dentistry,Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada
Seyed Vahid Malek Hosseini Private Practice, Yasouj, Iran
Negin Karimi Department of Orthodontic, School of Dentistry, Kerman University of Medical Science, Kerman, Iran

DOI: https://doi.org/10.18502/fid.v21i33.16436

Keywords: Casein Phosphopeptide-Amorphous Calcium Phosphate Nanocomplex; Tricalcium Phosphate; Tooth Remineralization

Abstract

Objectives: The main purpose of this study was to compare the remineralizing effects of casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF) and tricalcium phosphate (TCP) on artificially induced enamel white spot lesions (WSLs).

Materials and Methods: In this in vitro study, 45 sound extracted premolars were immersed in a demineralizing solution (pH=4.5) for 96 hours, and were randomly divided into 3 groups of TCP, MI Paste Plus, and control. They were exposed to the remineralizing agents for 5 minutes once a day for 30 days. After mounting the teeth in resin blocks and polishing, they underwent a microhardness test at 3 different depths from the enamel surface. Data were analyzed by Prism software, two-way ANOVA, and Tukey’s test (α=0.05).

Results: The volume percentage of mineral content (VPM) was significantly different among the three groups at 30-, 60- and 90µm depths (P<0.0001). At 30µm depth, CPP-ACPF was significantly more effective than TCP (P<0.0001). At 60- and 90µm depths, there was no significant difference between CPP-ACPF and TCP (P>0.05).

Conclusion: Both CPP-ACPF and TCP had significant efficacy for remineralization of artificially induced enamel WSLs under in vitro conditions.