Antimicrobial Efficacy of Saline, Chlorhexidine, and Zataria  Multiflora and Mentha Piperita Essential Oils in Root Canal  Irrigation of Primary Molars

Edris  Pordel; Masoud  Kiani; Ahmad  Jafari; Ali Reza  Heidari; Ronak  Bakhtiari

doi:10.18502/fid.v21i19.15682

Edris Pordel Department of Pediatric Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
Masoud Kiani Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Ahmad Jafari Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Ali Reza Heidari Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Ronak Bakhtiari Department of Pathobiology, Division of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/fid.v21i19.15682

Keywords: Root Canal Irrigants; Tooth, Deciduous; Root Canal Therapy; Herbal Medicine

Abstract

Objectives: This study aimed to compare the antimicrobial efficacy of saline, 0.5% and 2% Zataria multiflora (Z. multiflora) essential oil, 0.5% and 2% Mentha piperita (M. piperita) essential oil, and 0.2% chlorhexidine (CHX) as root canal irrigants for primary molar teeth.

Materials and Methods: A total of 64 primary molars were used in this in vitro study. The teeth were randomly assigned to six groups (N=10). The root canals were prepared up to file #35, and all teeth were sterilized before contamination with Enterococcus faecalis (E. faecalis; ATCC 29212) suspension. After 48 hours of incubation, the root canals in each group were irrigated with the respective irrigants. Sterile paper points were then used to collect microbial samples from the root canals. A colony counter was used to count the number of colony-forming units (CFUs). Data were analyzed by SPSS version 20 (alpha=0.05).

Results: The colony count was significantly different among the groups (P 0.5% M. piperita > 0.2% CHX > 2% M. piperita > 0.5% Z. multiflora.

Conclusion: The current study showed the optimal antibacterial activity of 0.5% Z. multiflora essential oil and 2% M. piperita essential oil against E. faecalis, and indicated their possible efficacy for use as an irrigant for root canal irrigation of primary molars.