Comparison of the Antibacterial Effect of AH26, Adseal and Beta RCS Root Canal Sealers Against Enterococcus Faecalis, an in Vitro Study

Sholeh  Ghabraei; Hadi  Assadian; Hassan  Razmi; Mohammad  Saeid Sheikhrezaei; Sedigheh  Khedmat; Nazanin  Chitsaz; Zahra  Mohammadi; Faranak  Noori

doi:10.18502/fid.v21i5.14853

Sholeh Ghabraei Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Hadi Assadian Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Hassan Razmi Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Saeid Sheikhrezaei Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Sedigheh Khedmat Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Nazanin Chitsaz Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Zahra Mohammadi Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Faranak Noori Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.18502/fid.v21i5.14853

Keywords: Anti-Bacterial Agents, Bacteria, Epoxy resin-based root canal sealer, Root Canal Filling Materials

Abstract

Objectives: Antibacterial activity against endodontic pathogens is a desirable feature for root canal sealers. The objective of this study was to compare the antibacterial effect of three resin-based endodontic sealers (AH26, Adseal, and Beta RCS) against Enterococcus faecalis in vitro.

Materials and Methods: The antibacterial properties of the sealers were assessed against E. faecalis using agar diffusion test (ADT) for fresh state (N=10) and direct contact test (DCT) for freshly-mixed and set states of the materials (N=10). In ADT, the diameter of the zones of inhibition was measured after 24h of contact. In DCT, the colony-forming units of the bacteria were counted after 30 minutes and 180 minutes of exposure. The results were analyzed with two-way ANOVA and independent sample t-test. P<0.05 was considered significant.

Results: Regarding DCT results, all test materials indicated an antibacterial effect, both in freshly-mixed and set states. The highest antibacterial effect was related to Adseal, whereas the lowest was observed in Beta RCS. There was a significant difference between all study groups (different sealers, setting states, and contact times; P<0.001), except for freshly-mixed AH26 and Adseal at 180 minutes (P>0.05). According to ADT, AH26 and Adseal represented the widest and the smallest inhibition zones, respectively (P<0.001).

Conclusion: Within the limitations of this in vitro study, AH26, Adseal, and Beta RCS showed antibacterial effects against E. faecalis in both freshly-mixed and set states. The antibacterial effect increased over time in all of the studied sealers.