Antifungal Effect of Ginger Essential Oil Spray on Candida albicans Adhering to Self-Cure Acrylic Plates

Zahra Azizi; Saeid Mahdavi Omran; Sedigheh Sheikhzadeh; Hemmat Gholinia; Samane Gharekhani

doi:10.18502/fid.v20i3.12279

Zahra Azizi Student Research Committee, Babol University of Medical Sciences, Babol, Iran
Saeid Mahdavi Omran Department of Medical Parasitology and Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
Sedigheh Sheikhzadeh Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Hemmat Gholinia Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Samane Gharekhani Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

DOI: https://doi.org/10.18502/fid.v20i3.12279

Keywords: Acrylic Resins; Candida albicans; Ginger; Oils, Volatile

Abstract

Objectives: The present study aimed to evaluate the efficacy of ginger essential oil spray for elimination of Candida albicans (C. albicans) adhering to self-cure acrylic plates.

Materials and Methods: In this experimental study, 120 self-cure acrylic discs were contaminated with C. albicans and randomly divided into four main groups: exposure to ginger essential oil, nystatin (positive control), distilled water (negative control), and no exposure. The minimum inhibitory concentration (MIC) of ginger oil and nystatin was determined by the microdilution test. The stability of C. albicans was determined by culturing the samples of treated acrylic plates and comparing the mean number of remaining colonies. Data were analyzed using the Kruskal-Wallis test followed by Dunn test with Bonferroni correction. P<0.05 was considered significant

Results: The MIC of ginger essential oil and nystatin was found to be 1560µg/mL and 4µg/mL, respectively. The differences between the mean count of C. albicans colonies before (10175±10730.25) and after the exposure to ginger essential oil (542.86±464.81) and nystatin (257.14±247.67) was statistically significant (P<0.001). The mean number of C. albicans colonies after spraying with nystatin was not significantly different compared with ginger essential oil (P=0.204). The efficacy of nystatin and ginger essential oil at each time was significantly more than distilled water (P<0.001). At 10 and 15min, there was no significant difference between nystatin and ginger essential oil groups (P=0.05).

Conclusion: Ginger essential oil spray was found to be a simple and effective method for elimination of C. albicans adhering to acrylic discs.