Periodontal Ligament Fibroblast Cell Viability Following Treatment with Different Concentrations of Green Tea, Aloe Vera and a Mixture of their Extracts

Seyed Hamed Alavi; Gita Rezvani; Mahmoud Nasr Esfahani; Fatemeh  Nobakht Lahrood

doi:10.18502/fid.v19i40.11901

Seyed Hamed Alavi Private Practice, Tehran, Iran
Gita Rezvani Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University, Tehran, Iran
Mahmoud Nasr Esfahani Department of Periodontology, Faculty of Dentistry, Shahed University, Tehran, Iran
Fatemeh Nobakht Lahrood Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

DOI: https://doi.org/10.18502/fid.v19i40.11901

Keywords: Fibroblasts; Periodontal ligament; Aloe; Tea; Cell survival

Abstract

Objectives: Various studies have identified green tea and Aloe vera as a suitable medium for avulsed teeth. The aim of this study was to evaluate and compare the viability of periodontal ligament (PDL) fibroblasts following treatment with the extracts of these two plants and their mixture.

Materials and Methods: Human PDL fibroblasts were purchased and treated with different concentrations of Aloe vera, green tea, and a combination of these two extracts. Hank’s balanced salt solution and culture medium were employed as positive and negative controls, respectively. Viability was assessed using the MTT assay. Two-way ANOVA and post-hoc tests were used for statistical analysis (P<0.05).

Results: There was a significant difference in PDL fibroblast viability between different concentrations of the extracts. Higher concentrations of green tea and the combination of the two extracts significantly increased cell viability. Higher concentrations of Aloe vera had the least positive effect on maintaining the viability of these cells.

Conclusion: If confirmed by further studies, the combination of Aloe vera and green tea extracts might be considered as a suitable media for different purposes like storing avulsed teeth.