Antimicrobial Resistance Pattern of Pigmented and Non-Pigmented Pseudomonas aeruginosa Isolated from Urine in a Tertiary Care Hospital of North India

Romaan  Jallu; Asifa  Nazir; Tabindah  Jahan

doi:10.18502/jmb.v14i1.21097

Romaan Jallu Department of Microbiology, Government Medical College, Srinagar, Jammu and Kashmir. India.
Asifa Nazir Department of Microbiology, Government Medical College, Srinagar, Jammu and Kashmir. India.
Tabindah Jahan Department of Microbiology, Amrita Hospital, Faridabad, Uttar Pradesh, India.

DOI: https://doi.org/10.18502/jmb.v14i1.21097

Keywords: Antimicrobial susceptibility testing, Pigment production, Pseudomonas, Urinary tract infection.

Abstract

Background: Pseudomonas spp is a clinically significant opportunistic pathogen frequently implicated in urinary tract infections. Differences in pigment production may indicate pathogenic variations and aid in optimizing treatment strategies.

Methods: A total of 4208 samples were processed according to standard microbiological techniques. Pigment production was enhanced on nutrient agar and antimicrobial sensitivity was performed according to CLSI guidelines. This data was then analyzed, noted and reported.

Results: 62 samples of Pseudomonas spp. were isolated, 29 (46.8%) samples did not produce any pigment, 19(30.6%) were green pyocyanin producers and 14 (22.6%) produced the pigment pyoverdine. Strains producing pigment were overall more resistant to tested antibiotics than strains not producing pigment. Pyoverdine producing strains demonstrated most resistance, but pyocyanin producing strains showed better sensitivity.

Conclusion: The findings underscore the need for routine pigment-based differentiation in clinical microbiology laboratories, as it may offer an early clue to resistance patterns and guide more effective empirical therapy