Isolation and Characterization of Isolates of Bacillus cereus Group and their Comparative Antibiotic Susceptibility Testing against Oxytetracycline

Kirti  Saini; Anil Kumar  Teotia; Prasad  Thota; Ajay Chandrakant  Lagashetti; Manoj  Kumar Pandey; Ajayendra  Kumar Keshari; Sanjay  Mendiratta; Meenakshi  Dahiya; Vivekanandan  Kalaiselvan

doi:10.18502/jmb.v14i1.21096

Kirti Saini Institute of Applied Medicine and Research, Duhai, 9th Km. Stone, Delhi-Meerut Road, Ghaziabad-201206, UP, India.
Anil Kumar Teotia Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Prasad Thota Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Ajay Chandrakant Lagashetti Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Manoj Kumar Pandey Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Ajayendra Kumar Keshari Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Sanjay Mendiratta Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Meenakshi Dahiya Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.
Vivekanandan Kalaiselvan Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India.

DOI: https://doi.org/10.18502/jmb.v14i1.21096

Keywords: Antibiotic resistance, Bacillus cereus, Characterization, Oxytetracycline, Susceptibility.

Abstract

Background: Bacillus cereus is a very important organism widely used for microbiological assaysof pharmaceutical products especially in antibiotic susceptibility testing. The increasing emergence ofantibiotic-resistant bacterial strains has emphasized the need for effective antibiotic susceptibilitytesting of pathogens for the management of patient’s health.

Methods: The present study isolated of 5 strains of B. cereus (R-01, R-02, C-01, C-03 & SS-01)from different samples collected from Ghaziabad, Uttar Pradesh, India. Pure cultures were thencharacterized based on microscopy, cultural and biochemical characteristics and also compared withstandard reference strain B. cereus MTCC 430. Further, all the 5 isolates were subjected to MALDI-TOF analysis to confirm their identity. Finally, antimicrobial susceptibility of all the indigenousstrains was tested in comparison with B. cereus MTCC 430 using the agar well diffusion method atvarious concentrations of Oxytetracycline (1.6, 2.0, 2.5, 3.12, 3.91, 4.0, 8.0, 12.0, & 16.0 μg/mL).

Results: Morphological, cultural and biochemical characteristics confirmed the identity of all thebacterial isolates as B. cereus which was further authenticated by MALDI-TOF analysis. Theantibiotic susceptibility tests revealed that out of 5 indigenous isolates, R-01 and R-02 were found tobe susceptible at all tested concentrations of Oxytetracycline; whereas C-01, C-03 and SS-01 werefound to be resistant at a concentration 2.5 μg/mL and below.

Conclusion: The antibiotics susceptibility test results displayed sensitivity profile of all 05 indigenousisolates of B. cereus against the Oxytetracycline and revealed that 02 isolates (R-01 and R-02) weremore sensitive to Oxytetracycline compared to others.