Characterization of E. coli Isolate and Assessment of its Antibiotic Susceptibility against Colistimethate Sodium

Komal Parashar; Anil Kumar Teotia; Prasad Thota; Ajay Chandrakant Lagashetti; Manoj Kumar  Pandey; Ajayendra Kumar  Keshari; Meenakshi Dahiya; Vivekanandan  Kalaiselvan

doi:10.18502/jmb.v13i4.20524

Komal Parashar Institute of Applied Medicine and Research, Duhai, 9th Km. Stone, Delhi-Meerut Road, Ghaziabad-201206, UP, India.
Anil Kumar Teotia Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India
Prasad Thota Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India
Ajay Chandrakant Lagashetti Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India
Manoj Kumar Pandey Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India
Ajayendra Kumar Keshari Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India
Meenakshi Dahiya Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India
Vivekanandan Kalaiselvan Indian Pharmacopoeia Commission, Ministry of Health and Family Welfare, Government of India, Sec-23, Raj Nagar, Ghaziabad-201002, UP, India

DOI: https://doi.org/10.18502/jmb.v13i4.20524

Keywords: Antimicrobial susceptibility, Characterization, Colistimethate sodium, E. coli.

Abstract

Background: The increasing application of Escherichia coli as a model organism in microbiologicalquality control highlights importance of E. coli due to their wide range of applications. However,rising antimicrobial resistance in E. coli produced a serious concern with respect to public healthglobally which eventually generated the need of screening of pathogens for their sensitivity andresistance profile against different antibiotics.

Methods: The present study isolated a bacterial strain of E. coli, SA-01 from Ghaziabad, UttarPradhesh, India which was then characterized based on morphology, microscopy, biochemical testingin comparison with standard reference strains of E. coli (ATCC 8739, ATCC 10536 and MTCC 448).The identity of the isolated strain was further confirmed by MALDI-TOF analysis. The antibioticsusceptibility of the isolated strain of E. coli (SA-01) was then assessed in comparison with E. coliATCC 10536 by cup plate method using different antibiotic doses of colistimethate sodium (1, 5, 10,20, 30, 40, 50, 100 and 461 μg/mL).

Results: Based on phenotypic and biochemical characteristics, isolated bacterial strain, SA-01 wasidentified as E. coli and was further authenticated by MALDI-TOF analysis. In the antibioticsusceptibility testing, the indigenous strain of E. coli (SA-01) has shown zone of inhibition similar toE. coli ATCC 10536.

Conclusion: Similar zone of inhibition compared to E. coli ATCC 10536 in the antibioticsusceptibility testing, revealed sensitivity profile of the indigenous strain of E. coli (SA-01) toColistimethate sodium suggesting its potential use in microbiological quality control methods.