Aptamer Biosensors: Rapid Heterogeneous Vancomycin-Intermediate Staphylococcus aureus Detection Solution

Hamid  Sadeghi; Farhad  Nikkhahi; Susan  Khanjani; Saeideh Gholamzadeh  Khoei; Mohammad Reza  Maleki; Masoumeh  Aslanimehr

doi:10.18502/jmb.v13i3.19514

Hamid Sadeghi Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Farhad Nikkhahi Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Susan Khanjani Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Saeideh Gholamzadeh Khoei Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.
Mohammad Reza Maleki Clinical Research Development Unit, Kowsar Hospital, Qazvin University of Medical Sciences, Qazvin, Iran
Masoumeh Aslanimehr Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

DOI: https://doi.org/10.18502/jmb.v13i3.19514

Keywords: Aptamer, hVISA, Rapid detection, Staphylococcus aureus.

Abstract

Vancomycin is an essential glycopeptide antibiotic facing challenges from resistant bacterial strainssuch as vancomycin-intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA).Timely and precise identification of these strains is vital for efficient infection control. This reportexplores using aptamer-based biosensors as a novel solution for the rapid detection of hVISA.Aptamers, short, single-stranded nucleic acids, exhibit remarkable specificity and strong affinity fortheir targets, making them outstanding candidates for biosensor creation. These biosensors offeraffordability, ease of customization, and swift performance, making them highly appropriate formedical settings. By utilizing electrochemical detection techniques, aptamer-based biosensorsfacilitate real-time tracking of vancomycin concentrations and may also contribute to the efficientdetection of hVISA, addressing urgent demands in infectious disease control.