Diagnosis of Chlamydia abortus by Isolation in Cell Culture and Real Time PCR in Aborted Sheep and Goats

Hossein  Esmaeili; Mona  Hamedi; Seyed Ahmad  Madani; Abbas  Barin; Fatemeh  Haji Agha Khiyabani

doi:10.18502/jmb.v12i4.17003

Hossein Esmaeili Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Mona Hamedi Department of Immunopathology, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research branch, Tehran, Iran.
Seyed Ahmad Madani Department of Animal Nutrition Plant Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abbas Barin Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Fatemeh Haji Agha Khiyabani Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

DOI: https://doi.org/10.18502/jmb.v12i4.17003

Keywords: Abortion, Cell culture, Chlamydia, Real-time PCR, Small ruminants.

Abstract

Background: Ovine enzootic abortion (OEA) caused by Chlamyidia abortus is one of the most important abortive disease in small ruminants. Diagnosis of Ovine enzootic abortion depends on the isolation and detection of the agent or its nucleic acid. The aim of the present study was to detect Chlamydia abortus using both isolation method and real-time PCR in Brucella free flocks in Iran.

Methods: Twenty-eight vaginal and conjunctival swab samples which were Chlamydia abortus seropositive, were selected from ewes and does with recent abortion. Then the samples were tested by real-time PCR and positive molecular samples were inoculated into McCoy cells.

Results: Using real-time PCR, 18 samples (64.3%) were positive and 7 (25%) of them were isolated in cell culture.

Conclusion: The present results indicate that Chlamydia can play a relatively significant role in the abortion in does and ewes in Iran. Although the isolation of Chlamydia abortus have 100% specificity, because of low sensitivity, time consuming, cost and high probability of contamination, it is not suitable for routine laboratory diagnosis. Therefore, applying real-time PCR which have high sensitivity and specificity is recommended.