Evaluation of Serum Levels of Anti-Helicobacter pylori IgG and IgA by ELISA Method in Patients with Gastric Ulcer in Chalous, Iran

Arezoo Taravat; Haniyeh Bashi Zadeh Fakhar; Babak  Shaghai; Fatameh Rouhollah

doi:10.18502/jmb.v12i1.15015

Arezoo Taravat Islamic Azad University, Chalus Branch Department of medical science, Chalous, Iran.
Haniyeh Bashi Zadeh Fakhar Department of Human Genetics, Science And Research Branch, Islamic Azad University, Tehran, Iran.
Babak Shaghai Department of Microbiology, Islamic Azad University, Chalous, Iran.
Fatameh Rouhollah Department of Molecular and Cellular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

DOI: https://doi.org/10.18502/jmb.v12i1.15015

Keywords: ELISA, Gastric Ulcer, Helicobacter pylori, IgA, IgG.

Abstract

Background: Considering the role of Helicobacter pylori in various diseases such as chronic gastritis, gastric ulcer and malignancies, this study was performed to evaluate the level of antiHelicobacter pylori antibodies in the serum of the population of Chalous and then its relationship with demographic characteristics.

Methods: A descriptive study was conducted in the department of Microbiology in a tertiary care teaching hospital during December 2019 to May 2021. All hospitalized patients with diarrhea, who had a history of exposure to antibiotic drugs and all patients with IBD were included in the study. Stool samples were collected from all patients with diarrhea who have been on antibiotic or anticancer drug during last 6weeks or previously diagnosed case. Patient’s demographic features and clinical details were collected from medical records.

Results: About 78% of people had IgA positive titer and about 48% had IgG positive titer. Serum IgA level in patients with bloating (P = 0.005) and in patients with nausea (P = 0.016) was statistically significant. Also, in patients studied there was a significant relationship between bloating (p = > 0.001), nausea (0.013) was seen with a serum IgG level .

Conclusion: Due to the high percentage of positive titers of anti-Helicobacter pylori antibodies, especially IgA in the sample population, it seems that the follow-up of positive serological titers will help to prevent and control the prevalence of Helicobacter pylori infection.