Combined Treatment with Adipose-Derived Stem Cells and Crocin Improved Cardiac Markers in Rat Model of Isoproterenol-Induced Myocardial Infarction

Rouzbeh  Mirzaei; Maryam  Radan; Mahin  Dianat; Khojasteh  Hoseinynejad; Fereshteh  Nejaddehbashi

doi:10.18502/jimc.v9i1.20495

Rouzbeh Mirzaei Department of Physiology, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Maryam Radan Department of Physiology, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Mahin Dianat Department of Physiology, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Khojasteh Hoseinynejad Department of Physiology, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Fereshteh Nejaddehbashi Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

DOI: https://doi.org/10.18502/jimc.v9i1.20495

Keywords: Crocin, Mesenchymal stem cells, Myocardial infarction, Rats

Abstract

Background: Stem cell transplantation after acute myocardial infarction is a new therapeutic strategy that has been claimed to restore heart function. Some studies have reported that the cardioprotective effects of crocin are related to the regulation of the antioxidant enzymes activity and cardiac biomarkers. Accordingly, the aim of this study was to investigate the effect of the combined treatment with mesenchymal stem cells derived from adipose tissue and crocin in the isoproterenol-induced myocardial infarction model.

Methods: Forty rats were randomly divided into the control, acute myocardial infarction, acute myocardial infarction plus cellular therapy, acute myocardial infarction plus cellular therapy and crocin, and the group receiving crocin. Cardiac damage biomarkers and oxidative stress indexes such as, Malondialdehyde (MDA) and TACrolimus (TAC) levels were evaluated in all the groups.

Results: A significant elevation in serum concentrations of troponin T, creatine kinase, lactate dehydrogenase, and alanine aminotransferase were observed as the primary indicators of cardiac injury in the ischemia model group when contrasted with the control rats. These findings correlated with a marked reduction in total antioxidant capacity and a significant rise in MDA levels in the group treated with Isoproterenol compared to the control group. The administration of mesenchymal stem cells in conjunction with crocin demonstrated improvements in cardiac biomarkers, which were associated with a decrease in MDA levels and an increase in total antioxidant capacity.

Conclusion: Based on the results of this study, antioxidant compounds along with cell therapy is suggested as an effective therapeutic strategy in ischemic heart damage. Cellular and molecular studies are necessary to identify the exact mechanism of effectiveness of this therapeutic strategy.